Cat. #161756
MCF10A EditR (Constitutive Cas9 expression) cell line
Cat. #: 161756
Availability: 8-10 weeks
Organism: Human
Tissue: Breast
Disease: Fibrocystic disease
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Corrado Santocanale
Institute: National University of Ireland Galway
Primary Citation: Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MCF10A EditR (Constitutive Cas9 expression) cell line
- Research fields: Cancer
- Parental cell: MCF10A
- Organism: Human
- Gender: Female
- Tissue: Breast
- Disease: Fibrocystic disease
- Growth properties: Adherent
- Model description: CRISPR
- Crispr: Yes
- Products or characteristics of interest: CRISPR drug screening
- Description: MCF10AEditR cells that stably express Cas9 nuclease. The cell line is used to perform a CRISPR-Cas9 genome-wide screen to identify genes that, when lost, promote the proliferation of cells treated with sub-efficacious doses of a CDC7i
- Application: SDS-PAGE and western blotting
- Production details: MCF10AEditR cells that stably express Cas9 nuclease were generated by lentiviral transduction of the Edit-R Cas9 plasmid and blasticidin-mediated selection.
- Biosafety level: 1
Applications
- Application: SDS-PAGE and western blotting
Handling
- Volume: Frozen
- Growth medium: DMEM supplemented with 5% Horse serum, 100 ng/ml cholera toxin, 10 ?g/ml insulin, 20 ng/ml epidermal growth factor (Peprotech), 500 ng/ml hydrocortisone, 50 U/ml penicillin and 50 ?g/ml Streptomycin
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Cultured in antibiotics: Penicillin / streptomycin
References
- Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864
