KPAR-G12C Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: KPAR-G12C Cell Line
• Alternate name:  
• Cancer: Lung cancer
• Research fields: Cancer
• Organism: Mouse
• Tissue: Lung
• Donor:  
• Model: Mutant
• Model description: A derivative from the KPAR cells, where the mutation Asp in position 12 from KRAS (G12D) was mutated to Cys (G12C) using prime editing.
• Crispr: No
• Products or characteristics of interest:  
• Description: Mouse models of mutant KRAS-driven lung cancer with an elevated tumour mutational burden by expressing the human DNA cytosine deaminase, APOBEC3B, to mimic the mutational signature seen in human lung cancer. The ability of KRAS/KRASG12 inhibitors to cause regression of KRASG12C expressing tumours was markedly potentiated by the adaptive immune system.
• Production details: A derivative from the KPAR cells, where the mutation Asp in position 12 from KRAS (G12D) was mutated to Cys (G12C) using prime editing.
• Biosafety level: 1
• Recommended controls:  

Handling

• Passage number:  
• Growth medium: DMEM media supported with 10% FBS, glutamine and penicillin/streptomycin
• Temperature:  
• Atmosphere:  
• Shipping conditions: Dry ice
• Storage medium:  
• Storage conditions: Liquid Nitrogen
• Initial handling information:  
• Subculture routine:  
• Cultured in antibiotics: Penicillin, Streptomycin
• Str profiling:  

Related Tools

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References

• Boumelha et al. 2022. Cancer Research. 82(19):3435-3448. PMID: 35930804.

Documentation