Immortalised Human Melanocyte [PIG1] Cell Line

An immortalised human melanocyte model used to study melanocyte biology, pigmentation pathways, and therapeutic target discovery. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Pranab K Das Amsterdam University
SKU: 154099
Product description: Immortalized human melanocyte cell line established from neonatal foreskin by introduction of retroviral construct carrying a geneticin resistance gene and containing HPV16E6E7 open reading frames.
Gender: Male
Conditional: No
Production details: Melanocyte cultures were established from neonatal foreskin using standard methods. Melanocytes from passage 12 were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In addition the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.
Cellosaurus ID: CVCL_S410
Parental cell line: Human foreskin
Cat. #: 154099
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Skin cancer
Research Fields: Cancer;Cell biology
Organism: Human
Tissue: Neonatal foreskin
Gender: Male
Model: Immortalised Line
Growth properties: Cells were routinely passaged 1:4 at confluency
Primary citation: Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Ham’s F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100 ug/ml streptomycin
Subculture routine: Cells were routinely passaged 1:4 at confluency
Mycoplasma free: Yes
Biosafety level: 1
References:

Ivanova et al. 2008. In Vitro Cell Dev Biol Anim. 44(8-9):385-95. PMID: 18594937

Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834

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