Cat. #161930
HT-3 Cell Line
Cat. #: 161930
Availability: 8-10 weeks
Tissue: Cervical tissue
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jorgen Fogh
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. PMID: 327080.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HT-3 Cell Line
- Cancer: Gynaecologic cancer
- Gender: Female
- Tissue: Cervical tissue
- Donor: This cell line was established in 1963 from a metastatic site (lymph node) in a 53-year-old Caucasian female.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: (P147) hypotriploid to hypertriploid (+A, +B, +C, +D, +F, -E). Modal number = 65 to 66 with abnormalities including dicentrics, acrocentric fragments, minutes, translocations, rings, secondary constrictions, breaks, pulverizations and large subtelocentric and large submetacentric markers. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1
- Description: HT-3 is a human cervical carcinoma cell line that grows in adherent culture.
- Application: 3D cell culture, Cancer research
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood type A; Rh+
Applications
- Application: 3D cell culture, Cancer research
Handling
- Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 15%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: X
References
- Rodan et al. 1987. Cancer research. 47(18). PMID: 3040234.
- Fathima et al. 2023. Cureus. 15(11). PMID: 38149135.