#161930

HT-3 Cell Line

Cat. #161930

HT-3 Cell Line

Cat. #: 161930

Availability: 8-10 weeks

Tissue: Cervical tissue

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jorgen Fogh

Institute: Memorial Sloan-Kettering Cancer Center (MSK)

Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. PMID: 327080.

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: HT-3 Cell Line
  • Cancer: Gynaecologic cancer
  • Gender: Female
  • Tissue: Cervical tissue
  • Donor: This cell line was established in 1963 from a metastatic site (lymph node) in a 53-year-old Caucasian female.
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Products or characteristics of interest: Karyotype: (P147) hypotriploid to hypertriploid (+A, +B, +C, +D, +F, -E). Modal number = 65 to 66 with abnormalities including dicentrics, acrocentric fragments, minutes, translocations, rings, secondary constrictions, breaks, pulverizations and large subtelocentric and large submetacentric markers. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1
  • Description: HT-3 is a human cervical carcinoma cell line that grows in adherent culture.
  • Application: 3D cell culture, Cancer research
  • Biosafety level: 1

Target Details

  • Target: Antigen expression: Blood type A; Rh+

Applications

  • Application: 3D cell culture, Cancer research

Handling

  • Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 15%.
  • Temperature: 37° C
  • Atmosphere: 5% CO2 in air
  • Shipping conditions: Dry Ice
  • Storage medium: Complete growth medium supplemented with 5% DMSO
  • Str profiling: Amelogenin: X

References

  • Rodan et al. 1987. Cancer research. 47(18). PMID: 3040234.
  • Fathima et al. 2023. Cureus. 15(11). PMID: 38149135.