#153186

HCT 116 p300 KO Rescue Cell Line

Please Enter Your Email ID
Email ID

Cat. #153186

HCT 116 p300 KO Rescue Cell Line

Cat. #: 153186

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Tissue: Colon

Disease: Cancer

Model: Knock-In

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Carlos Caldas

Institute: Cancer Research UK, Cambridge Institute

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HCT 116 p300 KO Rescue Cell Line
  • Cancer: Carcinoma
  • Cancers detailed: Carcinoma
  • Research fields: Cancer;Cell biology;Drug development
  • Tool sub type: Continuous
  • Parental cell: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Growth properties: Adhesion properties; invasion; migration
  • Model: Knock-In
  • Conditional: Yes
  • Description: This p300 rescue cell line was generated from p300 KO Clone F5.The HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration. Cell lines available are p300 WT (HCT116), p300 KO Clone D10 (HCT116), p300 KO Clone F5 (HCT116), p300 KO Clone F2 (HCT116). Experiments conducted should be using at least 2 of the 3 KO cell lines. The best 2 KO clones are D10 and F5. Anyone requesting the cell line should use the parental cell line that the KOs were generated from. The p300 KO lines demonstrate an aggressive cancer phenotype in vitro, with loss of cell-cell adhesion, defects in cell-matrix adhesion, and increased migration through collagen matrices. p300 is a transcriptional cofactor involved in regulating multiple cellular processes including cell cycle regulation, proliferation, differentiation, apoptosis, DNA damage repair and adhesion properties. Somatic inactivating mutations of p300 are associated with several cancers including breast, colorectal and gastric cancers.
  • Biosafety level: 1
  • Additional notes: CBP was not manipulated in this cell line
  • Recommended controls: HCT 116 parental line
  • Cellosaurus id: CVCL_HG30

Target Details

  • Target: p300

Applications

  • Application notes: CBP was not manipulated in this cell line

Handling

  • Format: Frozen
  • Growth medium: McCoy's 5A medium + 10% FCS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: HCT 116 p300 WT Cell Line ; HCT 116 p300 KO [F5] Cell Line ; HCT 116 p300 KO [F2] Cell Line ; HCT 116 p300 KO [D10] Cell Line

References

  • ?-HPV 5 and 8 E6 disrupt homology dependent double strand break repair by attenuating BRCA1 and BRCA2 expression and foci formation.
  • Wallace et al. 2015. PLoS Pathog. 11(3):e1004687. PMID: 25803638.
  • Iyer et al. 2007. Oncogene. 26(1):21-9. PMID: 16878158.
  • Bundy et al. 2006. Cancer Res. 66(15):7606-14. PMID: 16885360.
  • Metabolic consequences of p300 gene deletion in human colon cancer cells.
  • p300 is required for orderly G1/S transition in human cancer cells.
  • Krubasik et al. 2006. Br ...