Cat. #151479
HCT 116 p300 KO [D10] Cell Line
Cat. #: 151479
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Colon
Disease: Cancer
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Carlos Caldas
Institute: Cancer Research UK Cambridge Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HCT 116 p300 KO [D10] Cell Line
- Cancer: Carcinoma
- Cancers detailed: Carcinoma
- Research fields: Cancer;Cell biology;Drug development
- Tool sub type: Continuous
- Parental cell: HCT 116
- Organism: Human
- Tissue: Colon
- Disease: Cancer
- Growth properties: Adhesion properties; invasion; migration
- Model: Knock-Out
- Description: The HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration. Cell lines available are p300 WT (HCT116), p300 KO Clone D10 (HCT116), p300 KO Clone F5 (HCT116), p300 KO Clone F2 (HCT116). Experiments conducted should be using at least 2 of the 3 KO cell lines. The best 2 KO clones are D10 and F5. Anyone requesting the cell line should use the parental cell line that the KOs were generated from. The p300 KO lines demonstrate an aggressive cancer phenotype in vitro, with loss of cell-cell adhesion, defects in cell-matrix adhesion, and increased migration through collagen matrices. p300 is a transcriptional cofactor involved in regulating multiple cellular processes including cell cycle regulation, proliferation, differentiation, apoptosis, DNA damage repair and adhesion properties. Somatic inactivating mutations of p300 are associated with several cancers including breast, colorectal and gastric cancers. CBP was not manipulated in this cell line.
- Biosafety level: 1
- Additional notes: CBP was not manipulated in this cell line
- Recommended controls: HCT 116 parental line
- Cellosaurus id: CVCL_HG27
Target Details
- Target: p300
Handling
- Format: Frozen
- Growth medium: McCoy's 5A medium + 10% FCS
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: -20° C
- Mycoplasma free: Yes
Related Tools
- Related tools: HCT 116 p300 WT Cell Line ; HCT 116 p300 KO [F5] Cell Line ; HCT 116 p300 KO [F2] Cell Line ; HCT 116 p300 KO Rescue Cell Line
References
- Wallace et al. 2015. PLoS Pathog. 11(3):e1004687. PMID: 25803638.
- ?-HPV 5 and 8 E6 disrupt homology dependent double strand break repair by attenuating BRCA1 and BRCA2 expression and foci formation.
- Iyer et al. 2007. Oncogene. 26(1):21-9. PMID: 16878158.
- Bundy et al. 2006. Cancer Res. 66(15):7606-14. PMID: 16885360.
- Metabolic consequences of p300 gene deletion in human colon cancer cells.
- p300 is required for orderly G1/S transition in human cancer cells.
- Krubasik et al. 2006. Br ...
