BRCA2 c.156_157insAlu iPSC line (2041) cell line

Human iPSC disease model carrying the pathogenic BRCA2 c.156_157insAlu founder mutation associated with hereditary breast and ovarian cancer syndrome (HBOC). This model may be useful for studying BRCA2-associated hereditary cancer biology, DNA repair defects and early disease mechanisms in human-derived cellular systems.

Contributors

Inventor Institute
Maria Carmo-Fonseca ; Simao Teixeira da Rocha Instituto de Medicina Molecular Joao Lobo Antunes
Cat. #: 160739
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Fibroblast
Gender: Male
Model: Cancer Model
Product description: BRCA2-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA2-associated tumors, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA2 mutant hereditary cancers.
Gender: Male
CRISPR: Yes
Conditional: No
Production details: Reprogramming of human induced pluripotent stem cells from skin biopsy a female donor heterozygous for the BRCA2 mutation c.156_157insAlu.
Parental cell line: Human fibroblasts
Disease: Hereditary breast cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3-4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 C in a humidified 5% CO2 incubator.
Recommended controls: No
Mycoplasma free: Yes
References:

Silva TP et al. 2021. Stem Cell Res. PMID: 34087993.

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