Instituto de Medicina Molecular Jo?Âo Lobo Antunes
Cat. #:
160739
Unit size:
1×10^6 cells / vial
Research Fields:
Cancer;Genetics;Stem cell biology
Organism:
Human
Tissue:
Fibroblast
Model:
Cancer Model
Product description:
BRCA2-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA2-associated tumors, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA2 mutant hereditary cancers.
Conditional:
No
Production details:
Reprogramming of human induced pluripotent stem cells from skin biopsy a female donor heterozygous for the BRCA2 mutation c.156_157insAlu.
Parental cell line:
Human fibroblasts
disease:
Hereditary breast cancer
Format:
Frozen
Shipping conditions:
Dry ice
Growth medium:
Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3ÄË?Â?Â4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 ÄË?ÂÄÂC in a humidified 5% CO2 incubator.
Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.
