Cat. #160739
BRCA2 c.156_157insAlu iPSC line (2041) cell line
Cat. #: 160739
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Fibroblast
Disease: Hereditary breast cancer
Model: Cancer Model
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Maria Carmo-Fonseca ; Sim?Âo Teixeira da Rocha
Institute: Instituto de Medicina Molecular Jo?Âo Lobo Antunes
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: BRCA2 c.156_157insAlu iPSC line (2041) cell line
- Research fields: Cancer;Genetics;Stem cell biology
- Parental cell: Human fibroblasts
- Organism: Human
- Tissue: Fibroblast
- Disease: Hereditary breast cancer
- Model: Cancer Model
- Conditional: No
- Description: BRCA2-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA2-associated tumors, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA2 mutant hereditary cancers.
- Production details: Reprogramming of human induced pluripotent stem cells from skin biopsy a female donor heterozygous for the BRCA2 mutation c.156_157insAlu.
- Recommended controls: No
Target Details
- Target: Hereditary breast and ovarian cancer
Handling
- Format: Frozen
- Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3ÄË?Â?Â4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 ÄË?ÂÄÂC in a humidified 5% CO2 incubator.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
