A2780ADR Cell Line

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Premium cryopreserved A2780ADR cells resistant to adriamycin for the purpose of transfection and conducting research on ovarian cancer.

Contributors

Inventor Institute
Timothy Ward National Cancer Institute
SKU: 152707
Product description: Premium cryopreserved A2780ADR cells resistant to adriamycin for the purpose of transfection and conducting research on ovarian cancer.
Alternate name: A2780 ADR; A2780/ADR; A2780-ADR; A2780Adr; A2780ADR Cells; adriamycin-resistant cell line
Conditional: Yes
Production details: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5×1,000 to 2×10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.
Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 11,14 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,9 vWA: 15,16
Cellosaurus ID: CVCL_1941
Parental cell line: A2780
Disease: Cancer
Cat. #: 152707
Provenance and Ownership: The A2780ADR Cell Line is owned by Cancer Research UK. CancerTools supplies the A2780ADR Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing A2780ADR Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Ovary
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Carcinoma
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x10,000cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
References:

Miller et al. RSC Adv. 2018. 8(69):39731-39734. PMID: 30713687.

Singh et al. Int J Pharm. 2015. 478(2):745-752. PMID: 25437111.

Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.

Hamilton et al. 1984. Semin Oncol. 11(3):285-298. PMID: 6385258.

Images

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A2780ADR Cell Line. 24 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

Citations And Reviews

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