Anti-RSV Phosphoprotein VP32 [3-5-18]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-RSV Phosphoprotein VP32 [3-5-18]
• Research fields: Microbiology
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Virus
• Host: Mouse
• Application: ELISA ; IF ; WB
• Description: This antibody reacts with the phosphoprotein from both human RS virus subgroups A and B as well as the phosphoprotein of bovine RS virus. The antibody is not reactive with the phosphoprotein of other member of Pneumovirus genus (specifically, pneumonia virus of mice). On the basis of the reactivity of this antibody and the 4-14 antibody (cat number: 151851) against the VP32 protein (by immunoblot and immunofluorescence) the existence of two antigenic types of human RS virus was identified (Gimenez et al., 1986). The phosphoprotein is located within the virion nucleocapsid. The phosphoprotein of RS virus subgroup A is slightly larger than the phosphoprotein of RS virus subgroup B. This difference in molecular weight was demonstrated by immunoblot using this antibody (Gimenez et al., 1986). This antibody is useful for the diagnosis of RS virus infection since the phosphoprotein is abundant which makes the detection assays using this antibody highly sensitive.
• Immunogen: Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100°C for 2 mins.
• Isotype: IgG1 kappa
• Myeloma used: P3X63Ag8.653
• Recommended controls: Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Lane 1: 3-5 antibody. Lane2: RSV convalescent human sera.Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells

Target Details

• Target: Human Respiratory Syncytial (RS) virus Phosphoprotein, VP32
• Target background: This antibody reacts with the phosphoprotein from both human RS virus subgroups A and B as well as the phosphoprotein of bovine RS virus. The antibody is not reactive with the phosphoprotein of other member of Pneumovirus genus (specifically, pneumonia virus of mice). On the basis of the reactivity of this antibody and the 4-14 antibody (cat number: 151851) against the VP32 protein (by immunoblot and immunofluorescence) the existence of two antigenic types of human RS virus was identified (Gimenez et al., 1986). The phosphoprotein is located within the virion nucleocapsid. The phosphoprotein of RS virus subgroup A is slightly larger than the phosphoprotein of RS virus subgroup B. This difference in molecular weight was demonstrated by immunoblot using this antibody (Gimenez et al., 1986). This antibody is useful for the diagnosis of RS virus infection since the phosphoprotein is abundant which makes the detection assays using this antibody highly sensitive.

Applications

• Application: ELISA ; IF ; WB

Handling

• Format: Liquid
• Concentration: 1mg/ml
• Unit size: 100 ug
• Storage buffer: Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Gimenez et al. 1984. Journal General Virology, 65: 963-71. PMID: 6202832 Gimenez et al. 1986. Journal General Virology, 67: 863-70. PMID: 3517224 Gimenez et al. 1987. Journal General Virology, 68, 1267-75. PMID: 3572364