Anti-RSV F Glycoprotein [4-15]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-RSV F Glycoprotein [4-15]
• Research fields: Microbiology
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: The protein molecular weights were determined by co-electrophoresis with standard protein markers obtained from Sigma, their locations are marked on the figure. See also: “Positive control”.
• Strain: Balb/c
• Reactivity: Virus
• Host: Mouse
• Application: ELISA ; IHC ; IF ; WB
• Description: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa. On the basis of the reactivity of this antibody and the antibodies: clone 4-14 (cat number: 151851) and clone 3-5-18 (cat number: 151850), the existence of two antigenic types of human RS virus was identified, Gimenez et al. (1986). This antibody reacts with the F protein from both RS virus group A and B. It does not cross-react with other RSV proteins.
• Immunogen: The immunogen was gradient purified and UV inactivated RSN2 virus (subgroup B). The procedure used to produce this antibody is described in Gimenez et al. (1984).
• Isotype: IgG1 kappa, faint IgG2b
• Myeloma used: P3X63Ag8.653
• Recommended controls: Validation of the monoclonal antibodies by immunoblotting (see figure): purified extracellular RSV was used as the antigen in two adjacent lanes and the virus proteins resolved by gel electrophoresis. The proteins were transferred to membrane. The proteins for one lane were incubated with the MAb to be validated and the other lane incubated with either convalescent human sera (HuS), containing antibodies to all the RSV proteins, or RSV antiserum raised in mice. Human sera containing antibodies to the RSV proteins were characterised by immunoblot using purified RS virus (PMDI 3572364). The second antibodies used were: goat anti-human immunoglobulin (lane: HuS) and goat anti mouse immunoglobulin (lane: Mab). The identity and molecular weights of the RSV proteins (VP) were well documented by the time the validation was done and are indicated in the figure. The methods used provided not only the identity of the virus protein reacting with the MAb but also its molecular weight. The validation of this MAbs is described in PMID 3517224. Validation of the monoclonal antibodies by indirect immunofluorescence (see figure): for the detection of surface antigen the cells were stained directly without fixation. Cells were fixed immediately before mounting on to microscope slides

Target Details

• Target: Respiratory Syncytial (RS) virus Fusion glycoprotein
• Molecular weight: The protein molecular weights were determined by co-electrophoresis with standard protein markers obtained from Sigma, their locations are marked on the figure. See also: “Positive control”.
• Target background: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa. On the basis of the reactivity of this antibody and the antibodies: clone 4-14 (cat number: 151851) and clone 3-5-18 (cat number: 151850), the existence of two antigenic types of human RS virus was identified, Gimenez et al. (1986). This antibody reacts with the F protein from both RS virus group A and B. It does not cross-react with other RSV proteins.

Applications

• Application: ELISA ; IHC ; IF ; WB

Handling

• Format: Liquid
• Concentration: 1mg/ml
• Unit size: 100 ug
• Storage buffer: Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Gimenez HB et al. J Gen Virol. 1986 May
• 67 ( Pt 5):863-70. PMID: 3517224 Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.