Cat. #151247
Anti-RNA polymerase II [CTD 4H8] mAb
Cat. #: 151247
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: RNA Polymerase II
Class: Monoclonal
Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
Reactivity: Human ; Saccharomyces cerevisiae
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jesper Svejstrup
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Primary Citation: Kristjuhan et al. 2002. Mol Cell. 10(4):925-933. PMID: 12419235.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-RNA polymerase II [CTD 4H8] mAb
- Alternate name: RNAPII antibody; 4H8 antibody; CTD 4H8 antibody
- Research fields: Genetics
- Clone: CTD 4H8
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human ; Saccharomyces cerevisiae
- Host: Mouse
- Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
- Description: Anti-RNA polymerase II (RNAPII), clone CTD 4H8 is mouse monoclonal antibody. CTD 4H8 recognises both hypo- and hyperphosphorylated RNAPII. CTD 4H8 is known to be reactive with the C-terminal domain (CTD) in humans and saccharomyces cerevisiae. RNAPII is a multi-subunit enzyme responsible for the transcription of protein-coding genes. The C-terminal domain (CTD) is a highly conserved domain unqiue to RNAPII, coordinating factors that regulate transcription initiation, elongation a...
- Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
- Recommended controls: HeLa cell line.
Target Details
- Target: RNA Polymerase II
- Tissue cell line specificity: HeLa cell line.
- Target background: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
Applications
- Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-Phospho RNA polII (Ser5) [CTD 8A7]
References
- Williamson et al. 2017. Cell. 168(5):843-855. PMID: 28215706.
- Pang et al. 2014. Oncogene. 33(31):4039-4049. PMID: 24141769.
- Seo et al. 2014. Nucleic Acids Res. 42(2):1016-25. PMID: 24163101.
- Keren-Shaul et al. 2013. PLoS One. 8(1):e53506. PMID: 23326444.
- Harrigan et al. 2011. J Cell Biol. 193(1):97-108. PMID: 21444690.
- Li et al. 2006. Mol Biol Cell. 17(9):3848-3859. PMID: 16807355.
- Yart et al. 2005. Mol Cell Biol. 25(12):5052-5060. PMID: 15923622.
- Engelhardt et al. 2005. J Virol...
