Anti-RNA polymerase II [CTD 4H8] mAb

CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.

Contributors

Inventor Institute
Jesper Svejstrup Cancer Research UK, London Research Institute: Clare Hall Laboratories
Cat. #: 151247
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Genetics
Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
Target: RNA Polymerase II
Reactivity: Human ; Saccharomyces cerevisiae
Clone: CTD 4H8
Host: Mouse
Class: Monoclonal
Primary citation: Kristjuhan et al. 2002. Mol Cell. 10(4):925-933. PMID: 12419235.
Alternate name: RNAPII antibody; 4H8 antibody; CTD 4H8 antibody
Product description: Anti-RNA polymerase II (RNAPII), clone CTD 4H8 is mouse monoclonal antibody. CTD 4H8 recognises both hypo- and hyperphosphorylated RNAPII. CTD 4H8 is known to be reactive with the C-terminal domain (CTD) in humans and saccharomyces cerevisiae. RNAPII is a multi-subunit enzyme responsible for the transcription of protein-coding genes. The C-terminal domain (CTD) is a highly conserved domain unqiue to RNAPII, coordinating factors that regulate transcription initiation, elongation a…
Conjugation: Unconjugated
Isotype: IgG1
Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Myeloma used: Sp2/0-Ag14
Target background: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Williamson et al. 2017. Cell. 168(5):843-855. PMID: 28215706.

Pang et al. 2014. Oncogene. 33(31):4039-4049. PMID: 24141769.

Seo et al. 2014. Nucleic Acids Res. 42(2):1016-25. PMID: 24163101.

Keren-Shaul et al. 2013. PLoS One. 8(1):e53506. PMID: 23326444.

Harrigan et al. 2011. J Cell Biol. 193(1):97-108. PMID: 21444690.

Li et al. 2006. Mol Biol Cell. 17(9):3848-3859. PMID: 16807355.

Yart et al. 2005. Mol Cell Biol. 25(12):5052-5060. PMID: 15923622.

Engelhardt et al. 2005. J Virol…

Images

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Monoclonal antibody 4H8. RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNA polymerase II (RNAP II) contains a highly conserved heptapeptide repeat; YSPTSPS. Monoclonal antibody 4H8 was obtained using synthetic peptide and specifically recognises the C-terminal domain of RNAP II.

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