Cat. #151315
Anti-PHD3 [EG188e/d5]
Cat. #: 151315
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Prolyl Hydroxylase 3 (PHD3)
Class: Monoclonal
Application: IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Helen Turley
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-PHD3 [EG188e/d5]
- Research fields: Cancer;Genetics;Metabolism;Tissue-specific biology
- Clone: EG188e/d5
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 27.3 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: IHC ; WB
- Description: PHD3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. It promotes cell death through a caspase-dependent mechanism
- Immunogen: Residues 1-100 of human PHD3
- Isotype: IgG1
- Myeloma used: P3/NS1/1-Ag4.1
- Recommended controls: ZR75 cells on hypoxic induction
Target Details
- Target: Prolyl Hydroxylase 3 (PHD3)
- Molecular weight: 27.3 kDa
- Tissue cell line specificity: ZR75 cells on hypoxic induction
- Target background: PHD3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. It promotes cell death through a caspase-dependent mechanism
Applications
- Application: IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
Related Tools
- Related tools: Anti-PHD1 [PHD112/G7] ; Anti-PHD2 [366G/76/3]
References
- Fujita et al. 2012. J Biol Chem. 287(47):39942-53. PMID: 22948157.
- Prolyl hydroxylase 3 (PHD3) modulates catabolic effects of tumor necrosis factor-a (TNF-a) on cells of the nucleus pulposus through co-activation of nuclear factor B (NF-B)/p65 signaling.
- Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.
- Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues.
- Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.
- Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.
- Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected].
- Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.
