Cat. #151143
Anti-p53 [Pab 1104]
Cat. #: 151143
Sub-type: Primary antibody
Unit size: 100 ug
Target: p53
Class: Monoclonal
Application: IHC ; IP
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lionel Crawford
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Primary Citation: Leppard et al. 1983. EMBO J. 2(9):1457-64. PMID: 11892796.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-p53 [Pab 1104]
- Cancer: Other
- Cancers detailed: Broadly Applicable
- Research fields: Apoptosis and autophagy;Cancer;Cell biology;Genetics
- Clone: Pab 1104
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 53 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IP
- Description: PAb1104 has distinct, intrinsic activities towards both p53 and SV40 large T-antigen. Only a subset of T-antigen is bound by PAb1104.
- Immunogen: A slice of gel containing p53 homogenised in PBS. The p53 was extracted from the human SV40-transformed fibroblast cell line SV80 and purified by immunoprecipitation and SDS gel electrophoresis.
- Isotype: IgM
- Myeloma used: P3/NS1/1-Ag4.1
- Recommended controls: MDA-MB-231 cell line
Target Details
- Target: p53
- Molecular weight: 53 kDa
- Tissue cell line specificity: MDA-MB-231 cell line
- Target background: PAb1104 has distinct, intrinsic activities towards both p53 and SV40 large T-antigen. Only a subset of T-antigen is bound by PAb1104.
Applications
- Application: IHC ; IP
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Nayak et al. 2007. Cancer Res. 67(12):5831-9. PMID: 17575151.
- Effect of a single nucleotide polymorphism in the murine double minute 2 promoter (SNP309) on the sensitivity to topoisomerase II-targeting drugs.
- Leppard et al. 1984. J Virol. 50(2):457-64. PMID: 6323746.
- An oligomeric form of simian virus 40 large T-antigen is immunologically related to the cellular tumor antigen p53.
- Leppard et al. 1983. EMBO J. 2(9):1457-64. PMID: 11892796.
- Monoclonal antibodies displaying a novel spe...
