Anti Nucleocapsid Protein of MERS CoV [7H6]

Monoclonal antibody which detects the nucleocapsid viral protein of MERS coronavirus.

Contributors

Inventor Institute
Yee Joo Tan A*STAR Accelerate Technologies Pte Ltd
Cat. #: 154123
Unit size: 100 ug
Research Fields: Apoptosis and autophagy;Cell biology;Microbiology
Application: ELISA ; IF ; IP ; WB
Target: Nucleocapsid Protein of MERS-CoV
Reactivity: Mouse
Host: Mouse
Class: Monoclonal
Alternate name: Anti-Nucleocapsid Protein of MERS CoV [7H6]
Product description: Monoclonal antibody which detects the nucleocapsid viral protein of MERS coronavirus. Background and Research Application The Middle East Respiratory Syndrome coronavirus (MERS-CoV) is an enveloped, single stranded, positive-sense RNA viruses of the order Nidovirlaes, family Coronaviridae, genus Coronavirus and lineage C of the betacoronavirus. One of the viral proteins encoded by this virus is the structural nucleocapsid (N) protein. The N protein is a phosphoprotein and the most expressed virus protein during infection. It is approximately 413 aa and possesses the N terminal and C-terminal functional domains separated by a linker and flanged at the ends by the terminal arms by intrinsically disordered regions (IDRs). The N protein primarily protects the genome of virus by self-associating with virus RNAs and encapsulating them through the formation of ribonucleic proteins (RNPs). However, the protein is a multifunctional protein with secondary functions essential for virus replication and pathogenesis by interacting with both virus and host proteins. The N protein interacts with non-structural proteins or the replicase-transcriptase complexes (RTCs) essential for RNA synthesis to produce both genomic and subgenomic RNAs. In addition, it interacts with the other structural proteins to facilitate virus assembly and release. The N protein also serves as a regulatory protein interacting with host proteins. It regulates several cellular processes including innate immune responses by acting as a viral suppressor of RNA silencing to suppress host RNA-interferencemediated antiviral responses. The N protein is post-transitionally modified through phosphorylation and ADP-ribosylation which play a role in modulating cellular activities such as cell cycle, translational inhibitory, immunity, inflammation, cell proliferation, apoptosis, regulation of transcription, mRNA stability and cellular stress responses.
Conjugation: Unconjugated
Isotype: IgG2b kappa
Immunogen: R9UM87
Immunogen Uniprot ID: R9UM87
Target background: Monoclonal antibody which detects the nucleocapsid viral protein of MERS coronavirus. Background and Research Application The Middle East Respiratory Syndrome coronavirus (MERS-CoV) is an enveloped, single stranded, positive-sense RNA viruses of the order Nidovirlaes, family Coronaviridae, genus Coronavirus and lineage C of the betacoronavirus. One of the viral proteins encoded by this virus is the structural nucleocapsid (N) protein. The N protein is a phosphoprotein and the most expressed virus protein during infection. It is approximately 413 aa and possesses the N terminal and C-terminal functional domains separated by a linker and flanged at the ends by the terminal arms by intrinsically disordered regions (IDRs). The N protein primarily protects the genome of virus by self-associating with virus RNAs and encapsulating them through the formation of ribonucleic proteins (RNPs). However, the protein is a multifunctional protein with secondary functions essential for virus replication and pathogenesis by interacting with both virus and host proteins. The N protein interacts with non-structural proteins or the replicase-transcriptase complexes (RTCs) essential for RNA synthesis to produce both genomic and subgenomic RNAs. In addition, it interacts with the other structural proteins to facilitate virus assembly and release. The N protein also serves as a regulatory protein interacting with host proteins. It regulates several cellular processes including innate immune responses by acting as a viral suppressor of RNA silencing to suppress host RNA-interferencemediated antiviral responses. The N protein is post-transitionally modified through phosphorylation and ADP-ribosylation which play a role in modulating cellular activities such as cell cycle, translational inhibitory, immunity, inflammation, cell proliferation, apoptosis, regulation of transcription, mRNA stability and cellular stress responses.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Aboagye et al. 2018. Biosci Rep. 38(5):. PMID: 30242057.

Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.

Related Tools for Antibodies

Cat. # Tool Name
151015 Anti-CyclinA [E70.1] Key Info View Tool
151016 Anti-CyclinA [E72.1] Key Info View Tool
151019 Anti-Mos [S3.1] Key Info View Tool
151034 Anti-HSVUL42 [13D11] Key Info View Tool
151037 Anti-Cdk1 [17 (A17)] Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.