#161667

Anti-mouse Notch1

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Cat. #161667

Anti-mouse Notch1

Cat. #: 161667

Sub-type: Primary antibody

Target: Notch1 protein

Class: Monoclonal

Application: Flow Cytometry, IP, WB, IHC-Fr, ICC

Reactivity: Human, Mouse.

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Laurie Milner

Institute: Fred Hutchinson Cancer Research Center

Primary Citation: Huppert et al.2000. Nature. 405[6789):966-70. PMID: 10879540

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-mouse Notch1
  • Research fields: Cell biology
  • Clone: Mn1a
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Reactivity: Human, Mouse.
  • Host: Mouse
  • Application: Flow Cytometry, IP, WB, IHC-Fr, ICC
  • Description: Antibody mN1A recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands.
  • Isotype: IgG1
  • Recommended controls: WB: HAP1 cell lysate Flow Cyt (Intra): SH-SY5Y cells, Jurkat cells.

Target Details

  • Target: Notch1 protein
  • Target background: Intracellular domain of Notch1 protein, mainly its activated form. The Notch genes encode single-pass transmembrane receptors that transduce the extracellular signals responsible for cell fate determination during several steps of metazoan development.

Applications

  • Application: Flow Cytometry, IP, WB, IHC-Fr, ICC
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)

Handling

  • Storage conditions: Shipped at 4° C. Upon delivery aliquot and store at -20° C. Avoid freeze / thaw cycles.

References

  • Huppert et al.2000. Nature. 405[6789):966-70.PMID: 10879540