Anti-MBP [BBMBP23.42]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-MBP [BBMBP23.42]
• Research fields: Genetics
• Tool sub type: Primary antibody
• Class: Monoclonal
• Purpose: Marker
• Conjugation: Unconjugated
• Molecular weight: MBP precursor polypeptide 43 kDa, mature MBP 42 kDa
• Reactivity: E.coli
• Host: Mouse
• Application: IF ; WB
• Description: The E. coli maltose binding protein (MBP) is a product of the malE gene and is part of two multicomponent systems that uptake and sense maltose and higher-order glucose polymers (e.g. maltodextrin). MBP acts in the periplasmic space where it binds with high affinity to maltose/maltodextrin which, in turn binds to (i) a transport apparatus that translocates the MBP ligand across the inner membrane to the cytoplasm and (ii) chemoreceptors causing activation of a signaling pathway that directs taxis toward maltose (1). MBP is widely used as an affinity tag for purification of recombinant proteins. The reversible and high-affinity binding of MBP to amylose resins enables recovery of recombinant proteins at high purity in a single purification step. The purification and elution are performed under mild conditions, preserving the activity of the recombinant protein. In addition, MBP might increase expression, proper folding, and solubility, of its fusion partner (2–4).
• Immunogen: E.coli maltose binding protein (MBP) fusion construct
• Immunogen uniprot id: P0AEX9
• Isotype: IgG2a kappa
• Recommended controls: MBP expressing construct in bacteria or mammalian cells

Target Details

• Target: Maltose Binding Protein
• Molecular weight: MBP precursor polypeptide 43 kDa, mature MBP 42 kDa
• Tissue cell line specificity: Mouse
• Target background: The E. coli maltose binding protein (MBP) is a product of the malE gene and is part of two multicomponent systems that uptake and sense maltose and higher-order glucose polymers (e.g. maltodextrin). MBP acts in the periplasmic space where it binds with high affinity to maltose/maltodextrin which, in turn binds to (i) a transport apparatus that translocates the MBP ligand across the inner membrane to the cytoplasm and (ii) chemoreceptors causing activation of a signaling pathway that directs t...

Applications

• Application: IF ; WB

Handling

• Format: Liquid
• Unit size: 100 ug
• Shipping conditions: Dry ice

References

• Kimple et al. 2013. Curr Protoc Protein Sci. 73:Unit 9.9. PMID: 24510596.
• Riggs et al. 2001. Curr Protoc Mol Biol. Chapter 16:Unit16.6. PMID: 18265133.
• Kapust et al. 1999. Protein Sci. 8(8):1668-74. PMID: 10452611.
• Boos et al. 1998. Microbiol Mol Biol Rev. 62(1):204-29. PMID: 9529892.