Anti-Mannan [LM21]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Mannan [LM21]
• Alternate name: ?(1-4) Mannan
• Research fields: Biochemistry
• Class: Monoclonal
• Conjugation: Unconjugated
• Host: Rat
• Description: How the diverse polysaccharides present in plant cell walls are assembled and interlinked into functional composites is not known in detail. It has been shown that molecular recognition of mannan polysaccharides present in intact cell walls is severely restricted. In secondary cell walls, mannan esterification can prevent probe recognition of epitopes/ligands, and detection of mannans in primary cell walls can be effectively blocked by the presence of pectic homogalacturonan. Masking by pectic homogalacturonan is shown to be a widespread phenomenon in parenchyma systems, and masked mannan was found to be a feature of cell wall regions at pit fields. Direct fluorescence imaging using a mannan‐specific carbohydrate‐binding module and sequential enzyme treatments with an endo‐Î?‐mannanase has confirmed the presence of cryptic epitopes and that the masking of primary cell wall mannan by pectin is a potential mechanism for controlling cell wall micro‐environments.
• Immunogen: Neoglycoprotein immunogen, prepared by coupling of mannopentaose (Man5) and digalactosylmannopentaose (Gal2Man5) oligosaccharides to bovine serum albumin (BSA) by reductive amination.
• Myeloma used: IR983F
• Recommended controls: IgM

Target Details

• Target: Î?-(1→4)-manno-oligosaccharides from DP2 to DP5.
• Target background: How the diverse polysaccharides present in plant cell walls are assembled and interlinked into functional composites is not known in detail. It has been shown that molecular recognition of mannan polysaccharides present in intact cell walls is severely restricted. In secondary cell walls, mannan esterification can prevent probe recognition of epitopes/ligands, and detection of mannans in primary cell walls can be effectively blocked by the presence of pectic homogalacturonan. Masking by pectic homogalacturonan is shown to be a widespread phenomenon in parenchyma systems, and masked mannan was found to be a feature of cell wall regions at pit fields. Direct fluorescence imaging using a mannan‐specific carbohydrate‐binding module and sequential enzyme treatments with an endo‐Î?‐mannanase has confirmed the presence of cryptic epitopes and that the masking of primary cell wall mannan by pectin is a potential mechanism for controlling cell wall micro‐environments.

Handling

• Format: Liquid
• Unit size: 100 ug
• Shipping conditions: Shipping at 4° C

References

• Marcus et al. 2010. Plant J. 64(2):191-203. PMID: 20659281.