Anti-Leg1 [1G1]

Leg1 is a novel protein of 361 amino acids. Preliminary data suggested that Leg1 (previously named as D12) is involved in early liver development. However, no detailed characterization of Leg1 has been reported thus far. There are two copies of leg1 in zebrafish, namely leg1a and leg1b. Both leg1a and leg1b are expressed in the […]

Contributors

Institute
A*STAR Accelerate Technologies Pte Ltd
Cat. #: 152643
Unit size: 100 ug
Application: IF
Target: Liver-enriched gene 1 (previously called D12)
Reactivity: Zebrafish
Clone: 1G1
Host: Mouse
Class: Monoclonal
Product description: Leg1 is a novel protein of 361 amino acids. Preliminary data suggested that Leg1 (previously named as D12) is involved in early liver development. However, no detailed characterization of Leg1 has been reported thus far. There are two copies of leg1 in zebrafish, namely leg1a and leg1b. Both leg1a and leg1b are expressed in the larvae and adult liver with leg1a being the predominant form. Knockdown of Leg1a or Leg1b by their respective morpholinos specifically targeting their 59-UTR each resulted in a small liver phenotype, demonstrating that both Leg1a and Leg1b are important for early liver development.
Conjugation: Unconjugated
Isotype: IgG1 kappa
Immunogen: GST-Leg 1 fusion protein
Myeloma used: Sp2/0-Ag14
Target background: Leg1 is a novel protein of 361 amino acids. Preliminary data suggested that Leg1 (previously named as D12) is involved in early liver development. However, no detailed characterization of Leg1 has been reported thus far. There are two copies of leg1 in zebrafish, namely leg1a and leg1b. Both leg1a and leg1b are expressed in the larvae and adult liver with leg1a being the predominant form. Knockdown of Leg1a or Leg1b by their respective morpholinos specifically targeting their 59-UTR each resulted in a small liver phenotype, demonstrating that both Leg1a and Leg1b are important for early liver development.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Chang et al. 2011. PLoS One. 6(8):e22910. PMID: 21857963

Images

View Gallery
Western Blot was performed on zebrafish embryo cells over-expressing D12 lysate (+) or without transfection to demonstrate endogenous expression (-)

Related Tools for Antibodies

Cat. # Tool Name
151023 Anti-IL12 [1-1D5] Key Info View Tool
151026 Anti-FCGR1 [10.1] mAb Key Info View Tool
151033 Anti-HSVUL42 [13C9] Key Info View Tool
151017 Anti-MBP [R29.6] Key Info View Tool
151038 Anti-Cytochrome P450 4A2, 4A3 [Clo4] Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.