This antibody doesn’t appear to work for western blotting as it recognizes the native protein.
| Inventor | Institute |
|---|---|
| Elizabeth A. Wayner | Fred Hutchinson Cancer Center |
| Cat. #: | 161662 |
|---|---|
| Tool sub type: | Primary antibody |
| Research Fields: | Cell biology |
| Application: | ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot |
| Target: | Integrin beta-1 (CD29), human |
| Reactivity: | Human |
| Clone: | P4C10 |
| Host: | Mouse |
| Class: | Monoclonal |
| Primary citation: | Carter et al. 1990. J Cell Biol. 110(4):1387-404. PMID: 1691191 |
| Product description: | MoAb P4C10 was developed to identify a new fibronectin receptor that is identical to the integrin receptor ?1. P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type I, fibronectin and laminin; also inhibits cell-cell adhesion. The epitope recognized by P4C10 is not trypsin sensitive. |
|---|---|
| Isotype: | IgG1 |
| Molecular weight: | Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa |
| Immunogen: | HT1080 human fibrosarcoma cell line |
| Target background: | integrin ?1 (CD29), human, anti-?1 mAb |
|---|
| Storage conditions: | For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing. |
|---|---|
| Shipping conditions: | Dry ice |
| References: |
Carter et al. 1990. J Cell Biol.111(6):3141-54.PMID: 2269668 Takadaet al. 1993. J Biol Chem. 268(23):17597-601.PMID: 7688727 Wayner et al.1993. Cell Biol.121(5):1141-52.PMID: 8501119 Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064 |
|---|
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