Anti-integrin a4 [P4G9]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-integrin a4 [P4G9]
• Research fields: Cell biology
• Clone: P4G9
• Tool sub type: Primary antibody
• Class: Monoclonal
• Molecular weight: 114.9 kDa
• Reactivity: Human
• Host: Mouse
• Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Description: MoAb P4G9 was developed to identify a new fibronectin receptor that is identical to the integrin receptor ?4/?1. P4G9 recognized epitopes on the ?4 subunit and completely inhibited the adhesion of peripheral blood and cultured T lymphocytes to a 38-kD tryptic fragment of plasma fibronectin containing the carboxy-terminal Heparin II domain and part of the type III connecting segment . The ligand in type III connecting segment (IIICS ) for ?4/?1was the CS-1 region previously defined as an adhesion site for melanoma cells. P4G9 stimulates aggregation of T cells as well.
• Immunogen: HT1080 human fibrosarcoma cell line
• Isotype: IgG2b

Target Details

• Target: Integrin alpha-4 (CD49d)
• Molecular weight: 114.9 kDa
• Target background: A fibronectin receptor that is identical to the integrin receptor ?4?1. CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit ?

Applications

• Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

• Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

References

• Wayner et al. 1989. Journal of Cell Biology. 109(3):1321-30. PMID: 2527858
• Schiffer et al.1995.J Biol Chem. 270(24):14270-3.PMID: 7782282
• Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064