#151171

Anti-HPV16 E1/E4 [TVG 402]

Cat. #151171

Anti-HPV16 E1/E4 [TVG 402]

Cat. #: 151171

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Human Papilloma Virus 16 early proteins 1 and 4 (HPV16 E1/E4)

Class: Monoclonal

Application: ELISA ; IHC ; IP ; WB

Reactivity: Human papilloma virus

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: University of Cambridge

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-HPV16 E1/E4 [TVG 402]
  • Cancer: Gynaecologic cancer
  • Cancers detailed: Cervical
  • Research fields: Cancer;Microbiology
  • Clone: TVG 402
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 10 kDa
  • Strain: Balb/c
  • Reactivity: Human papilloma virus
  • Host: Mouse
  • Application: ELISA ; IHC ; IP ; WB
  • Description: TVG 402 and TVG 403 were the first antibodies against HPV16 E1 & E4 and should complement those already available to E7 and L1 for the screening of frozen sections of clinical biopsies. They will be of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.
  • Immunogen: Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide.
  • Isotype: IgG1
  • Myeloma used: NS0

Target Details

  • Target: Human Papilloma Virus 16 early proteins 1 and 4 (HPV16 E1/E4)
  • Molecular weight: 10 kDa
  • Target background: The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer case. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 402 and TVG 403 were the first antibodies against HPV16 E1 & E4 and should complement those already available to E7 and L1 for the screening of frozen sections of clinical biopsies. They will be of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Applications

  • Application: ELISA ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Doorbar et al. 2000. J Virol. 74(21):10081-95. PMID: 11024137.
  • The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus.
  • Doorbar et al. 1992. Virology. 187(1):353-9. PMID: 1371027.
  • Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein.