Cat. #151225
Anti-HLADR beta chain 1 [DA2]
Cat. #: 151225
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Human Leukocyte Antigen DR ? chain 1 (1st domain, monomorphic, MHC class II)
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-HLADR beta chain 1 [DA2]
- Research fields: Immunology
- Clone: DA2
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; WB
- Description: Monoclonal antibody which binds all DR and DP antigens, with use in tissue and HLA typing.
- Immunogen: The membranes of LKT cells (a B lymphoblastoid cell line)
- Immunogen uniprot id: P01911
- Isotype: IgG1
- Myeloma used: P3X63Ag8
Target Details
- Target: Human Leukocyte Antigen DR ? chain 1 (1st domain, monomorphic, MHC class II)
- Target background: Monoclonal antibody which binds all DR and DP antigens, with use in tissue and HLA typing. Background and Research Application Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response. DA2 may be used for tissue typing. This antibody will bind all DR and DP antigens except DR7, and can aid with HLA typing.
Applications
- Application: ELISA ; FACS ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Brodsky et al. 1980. Tissue Antigens. 16(1):30-48. PMID: 6162228.
- Monoclonal antibodies to HLA--DRw determinants.
- Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
- Monoclonal antibodies for analysis of the HLA system.
