#161670

Anti-CFU-GM

Cat. #161670

Anti-CFU-GM

Cat. #: 161670

Sub-type: Primary antibody

Target: CFU-GM (granulocyte-macrophage colony-forming units)

Class: Monoclonal

Application: Indirect Immunofluorescent Staining, Fluorescence-Activated Cell Sorting

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Irwin Bernstein

Institute: Fred Hutchinson Cancer Research Center

Primary Citation: Andrews etal.1983. Blood. 62(1):124-32. PMID: 6190518

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-CFU-GM
  • Clone: T5A7
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Strain: Application Notes: They are potentially useful for depleting or enriching myeloid cell populations, including committed hematopoietic precursor cells. They provide probes to examine cell surface biochemical changes and eventually the responsible genetic alterations that occur during normal as well as malignant myeloid differentiation. They relate the cell surface phenotype of malignant myeloid cells to stages of normal myelocytic differentiation based on the expression of maturation-linked antigens.
  • Reactivity: Human
  • Host: Mouse
  • Application: Indirect Immunofluorescent Staining, Fluorescence-Activated Cell Sorting
  • Description: This antibody will react with both granulocytic and monocytic cells. It is potentially useful for depleting or enriching myeloid cell populations, including committed hematopoietic precursor cells.
  • Isotype: IgM

Target Details

  • Target: CFU-GM (granulocyte-macrophage colony-forming units)
  • Target background: Myeloid-associated determinants on committed myeloid stem cells and their progeny.

Applications

  • Application: Indirect Immunofluorescent Staining, Fluorescence-Activated Cell Sorting
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)

Handling

  • Storage conditions: For immediate use, short term storage at 4° C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20° C or -80° C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

References

  • Andrews etal.1983. Blood. 62(1):124-32.PMID: 6190518