Cat. #151173
Anti-CD45RO [UCH-L1]
Cat. #: 151173
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RO)
Class: Monoclonal
Application: FACS ; IHC ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Beverley
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CD45RO [UCH-L1]
- Alternate name: Protein Tyrosine Phosphatase; Receptor Type C; CD45 Antigen; CD45; L-CA; T2; Protein Tyrosine Phosphatase; Receptor Type; C Polypeptide; T2 Leukocyte Common Antigen; T2 Glycoprotein; EC 3.1.3.48; GP18; CD45R; B22; LCA; LY5
- Research fields: Cancer;Cell biology;Immunology;Tags and cell markers
- Clone: UCH-L1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 180 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: FACS ; IHC ; IP ; WB
- Description: Highly specific monoclonal antibody which can detect CD45RO, differentiating between subpopulations of T lymphocytes.
- Immunogen: Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
- Immunogen uniprot id: P08575
- Isotype: IgG2a
- Myeloma used: P3/NS1/1-Ag4.1
Target Details
- Target: Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RO)
- Molecular weight: 180 kDa
- Target background: CD45RO (UCHL1) is also known as protein tyrosine phosphatase receptor type C (PTPRC) or leukocyte common antigen. It is a transmembrane pan-leukocyte protein with tyrosine phosphatase activity and is involved in the regulation of signal transduction in haematopoiesis and the threshold of T cell antigen receptor (TCR) signalling.
Applications
- Application: FACS ; IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Dry ice
Related Tools
- Related tools: Anti-CD45RO, Recombinant [UCH-L1]
References
- Elgueta et al. 2019. Front Immunol. 10:981. PMID: 31118938.
- Moura et al. 2017. Cell Mol Immunol. 14(9):758-769. PMID: 26996067.
- Wu et al. 2015. Oncotarget. 6(30):28633-45. PMID: 26416419.
- Zlobec et al. 2013. J Transl Med. 11:104. PMID: 23627766.
- Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types.
- Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.
- Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.
- Ishii et al. 2001. Proc Natl Acad Sci U S A. 98(21):12138-43. PMID: 11593028.
- CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO.
- Ponsford et al. 2001. Clin Exp Immunol. 124(2):315-22. PMID: 11422210.
- Differential responses of CD45+ve T-cell subsets to MBP in multiple sclerosis.
- Smith et al. 1986. Immunology. 58(1):63-70. PMID: 2423439.
- Fn subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.
