Cat. #161652
Anti-CD44 [P1H5]
Cat. #: 161652
Sub-type: Primary antibody
Availability: 3-4 weeks
Target: CD44
Class: Monoclonal
Application: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Elizabeth A. WaynerWilliam Carter,
Institute: Fred Hutchinson Cancer Center
Primary Citation: Wayner et al.1987. Journal of Cell Biology.105(4):1873-84. PMID: 2822727
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CD44 [P1H5]
- Research fields: Cell biology
- Clone: P1H5
- Tool sub type: Primary antibody
- Class: Monoclonal
- Molecular weight: 125-145 kDa
- Reactivity: Human
- Host: Mouse
- Application: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
- Description: MoAb is a mouse monoclonal antibody raised against HT-1080. MoAb was developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. This antibody inhibited cell adhesion to type VI and I collagens only and not to fibronectin or laminin.
- Immunogen: HT1080 human fibrosarcoma cell line
- Isotype: IgG1
Target Details
- Target: CD44
- Molecular weight: 125-145 kDa
- Target background: CD44 is a hyaluronan cell adhesion molecule (HCAM), GP90 lymphocyte homing receptor, and class III collagen receptor (CRIII). Antibody bound and inhibited cell adhesion to type VI and I collagens (and bound type III, IV, and V collagens with no inhibition) only and not to fibronectin or laminin
Applications
- Application: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.
- Shipping conditions: Dry ice
References
- Wayner et al.1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727
- Carter et al.1990. J Cell Biol.111(6):3141-54.PMID: 2269668
- Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064
