Anti-CD44 [P1H5]

P1H5 precipitated a single cell surface protein. The data obtained from inhibition of cell attachment studies with class II antibodies strongly suggested that attachment to collagen was mediated by this class II receptor.

Contributors

Inventor Institute
Elizabeth A. WaynerWilliam Carter, Fred Hutchinson Cancer Center
Cat. #: 161652
Tool sub type: Primary antibody
Research Fields: Cell biology
Application: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
Target: CD44
Reactivity: Human
Clone: P1H5
Host: Mouse
Class: Monoclonal
Primary citation: Wayner et al.1987. Journal of Cell Biology.105(4):1873-84. PMID: 2822727
Product description: MoAb is a mouse monoclonal antibody raised against HT-1080. MoAb was developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. This antibody inhibited cell adhesion to type VI and I collagens only and not to fibronectin or laminin.
Isotype: IgG1
Molecular weight: 125-145 kDa
Immunogen: HT1080 human fibrosarcoma cell line
Target background: CD44 is a hyaluronan cell adhesion molecule (HCAM), GP90 lymphocyte homing receptor, and class III collagen receptor (CRIII). Antibody bound and inhibited cell adhesion to type VI and I collagens (and bound type III, IV, and V collagens with no inhibition) only and not to fibronectin or laminin
Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.
Shipping conditions: Dry ice
References:

Wayner et al.1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727

Carter et al.1990. J Cell Biol.111(6):3141-54.PMID: 2269668

Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064

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