Anti-CAR Whitlow Linker [1H5b]

The anti-CAR Whitlow linker peptide 1H5b clone has been developed to detect cells expressing Whitlow linker-containing CARs with different antigen specificities, including those harbouring the widely employed anti-CD19 FMC63-derived scFv as well as other scFvs, such as those targeting B-cell maturation antigen (BCMA) or CD33. It has been demonstrated to have a mean KD of […]

Contributors

Inventor Institute
Erik Kimble, Jocelyn Wright Fred Hutchinson Cancer Center
Cat. #: 162438
Unit size: 1 mg
Application: Flow cytometry
Target: Chimeric antigen receptor Whitlow scFv peptide linker
Clone: 1H5B
Host: Mouse
Class: Monoclonal
Primary citation: Kimble et al. 2025.J Immunother Cancer. 2025 Nov 18;13(11):e013123. PMID: 41253497
Product description: The anti‑CAR Whitlow linker peptide 1H5b clone is a murine monoclonal antibody that detects cells expressing Whitlow linker‑containing CARs with different antigen specificities, including those harbouring the FMC63‑derived anti‑CD19 scFv as well as other scFvs such as those targeting CD33. It has a mean KD of approximately 0.10 µM for FMC63‑Whitlow scFv protein and 0.11 µM for the Whitlow peptide, with no observable binding to the G4S4 peptide. In flow cytometry, 1H5b stains engineered T cells expressing FMC63‑ or 1H7‑Whitlow CARs, but not the corresponding FMC63‑ or 1H7‑G4S CARs.
Conjugation: Unconjugated
Isotype: IgG1
Immunogen: Synthetic Whitlow peptide
Target background: While CAR T-cell therapies have revolutionized the treatment of B-lineage malignancies, high-resolution tracking of CAR-engineered cells within the tumor microenvironment (TME) remains a significant technical challenge, particularly in archival formalin-fixed paraffin-embedded (FFPE) tissues. To address this, murine monoclonal antibodies (mAbs) have been developed to specifically target the Whitlow linker, a synthetic peptide commonly utilized in the scFv domains of multiple FDA-approved CAR products (e.g., axi-cel, liso-cel). Because this linker is absent in native human tissue and conserved across various antigen specificities, these mAbs provide a universal tool for the in situ identification, selection, and functional analysis of CAR-expressing cells. This methodology enables a more precise evaluation of CAR T-cell infiltration, persistence, and correlation with clinical outcomes or toxicities.

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