#161641

Anti-Botulinum neurotoxin monoclonal antibody [5BA2.3]

Cat. #161641

Anti-Botulinum neurotoxin monoclonal antibody [5BA2.3]

Cat. #: 161641

Sub-type: Primary antibody

Target: Clostridium botulinum neurotoxin A

Class: Monoclonal

Reactivity: Clostridium botulinum

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: UK Health Security Agency (UKHSA / PHE)

Tool Details
Target Details
Applications
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Botulinum neurotoxin monoclonal antibody [5BA2.3]
  • Clone: 5BA2.3
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Horseradish peroxidase (HRP)
  • Molecular weight: 150 kDa
  • Reactivity: Clostridium botulinum
  • Host: Mouse
  • Description: Clostridium botulinum neurotoxins are produced by the spore-forming bacteria with the same name and can lead to life-threatening botulism, having major implications for food safety. Botulinum toxins cause muscle paralysis by blocking acetylcholine, and this property of botulinum toxin A is used to treat several muscular and dermatological disorders. More recently, its popularity has extended in cancer therapy to provide analgesia in spastic conditions. The HRP-conjugated anti-Clostridium botulinum neurotoxin A antibody provides a valuable tool for target detection. This tool is suitable for researching the mechanisms and effects of the widely used Clostridium botulinum neurotoxin A, as well as dosing studies or quality control.
  • Isotype: IgG
  • Additional notes: HRP conjugation done with concentrated purified antibody at > 4 mg/mL using Peroxidase labelling kit (Roche)

Target Details

  • Target: Clostridium botulinum neurotoxin A
  • Target alternate names: Botulinum toxin A, bontoxilysin-A (BOTOX), BoNT/A
  • Molecular weight: 150 kDa
  • Target background: Clostridium botulinum neurotoxin A (BoNT A) is one of the seven serotypes of Clostridium botulinum toxins. BoNT A consists of an inactive 1296 AA long polypeptide chain of approximately 150 kDa which is cleaved by endogenous proteases. After cleavage, BoNT A becomes active in the form of a molecule with a light chain and a heavy chain. The light chain has protease activity, whilst the heavy chain containing a translocation domain and a two-units receptor binding domain. BoNT A binds to a corresponding receptor on the neuronal surface through its heavy chain, whilst the light chain cleaves a key component in exocytosis at the synaptic level, therefore blocking the release of acetylcholine and action potential transmission. Wheeler and Smith. 2013. Toxicology. 306: 124-146. PMID: 23435179 ; UniProt ID: P0DPI0

Applications

  • Application notes: Supplied at 1 mg/mL.

Handling

  • Storage buffer: PBS / 10 mM glycine pH 7.4 and 0.01% thimerosal
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles