Anti-Botulinum neurotoxin monoclonal antibody [5BA2.3]

Anti botulinum toxin antibody for neurotoxin detection

Contributors

Institute
UK Health Security Agency (UKHSA / PHE)
Cat. #: 161641
Tool sub type: Primary antibody
Research Fields: Cancer;Neurobiology
Target: Clostridium botulinum neurotoxin A
Reactivity: Clostridium botulinum
Clone: 5BA2.3
Host: Mouse
Class: Monoclonal
Product description: Clostridium botulinum neurotoxins are produced by the spore-forming bacteria with the same name and can lead to life-threatening botulism, having major implications for food safety. Botulinum toxins cause muscle paralysis by blocking acetylcholine, and this property of botulinum toxin A is used to treat several muscular and dermatological disorders. More recently, its popularity has extended in cancer therapy to provide analgesia in spastic conditions. The HRP-conjugated anti-Clostridium botulinum neurotoxin A antibody provides a valuable tool for target detection. This tool is suitable for researching the mechanisms and effects of the widely used Clostridium botulinum neurotoxin A, as well as dosing studies or quality control.
Additional notes: HRP conjugation done with concentrated purified antibody at > 4 mg/mL using Peroxidase labelling kit (Roche)
Conjugation: Horseradish peroxidase (HRP)
Isotype: IgG
Molecular weight: 150 kDa
Target background: Clostridium botulinum neurotoxin A (BoNT A) is one of the seven serotypes of Clostridium botulinum toxins. BoNT A consists of an inactive 1296 AA long polypeptide chain of approximately 150 kDa which is cleaved by endogenous proteases. After cleavage, BoNT A becomes active in the form of a molecule with a light chain and a heavy chain. The light chain has protease activity, whilst the heavy chain containing a translocation domain and a two-units receptor binding domain. BoNT A binds to a corresponding receptor on the neuronal surface through its heavy chain, whilst the light chain cleaves a key component in exocytosis at the synaptic level, therefore blocking the release of acetylcholine and action potential transmission. Wheeler and Smith. 2013. Toxicology. 306: 124-146. PMID: 23435179 ; UniProt ID: P0DPI0
Storage buffer: PBS / 10 mM glycine pH 7.4 and 0.01% thimerosal
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice

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