Anti-BARA/LIN-9

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-BARA/LIN-9
• Alternate name: BARA/LIN-9
• Research fields: Cell biology;Genetics
• Class: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Rabbit
• Application: WB ; FACS ; ELISA
• Description: LIN-9 regulates cell transformation and proliferation in mammalian cells by inhibiting DNA synthesis. LIN-9 is inhibited by the regulatory subunit of CDK4, cyclin D. Deletion of the first 84 amino acids of Mip/LIN-9 (Mip/LIN-9∆84) corrects the CDK4 null phenotype. Therefore, Mip/LIN-9, like the pocket proteins pRB, p107 and p130, is negatively regulated by CDK4. Moreover, the correction of the CDK4 null phenotype is accompanied by a restoration of the expression of genes such as E2F1, E2F3, and cyclin E suggesting that Mip/LIN-9 participates in the regulation of E2F target genes required for the G1/S transition.
• Immunogen: Synthetic peptide (amino acids 420–434)

Target Details

• Target: b-Chain Associated Regulator of Apoptosis (BARA)/LIN-9
• Target background: LIN-9 regulates cell transformation and proliferation in mammalian cells by inhibiting DNA synthesis. LIN-9 is inhibited by the regulatory subunit of CDK4, cyclin D. Deletion of the first 84 amino acids of Mip/LIN-9 (Mip/LIN-9∆84) corrects the CDK4 null phenotype. Therefore, Mip/LIN-9, like the pocket proteins pRB, p107 and p130, is negatively regulated by CDK4. Moreover, the correction of the CDK4 null phenotype is accompanied by a restoration of the expression of genes such as E2F1, E2F3, and cyclin E suggesting that Mip/LIN-9 participates in the regulation of E2F target genes required for the G1/S transition.

Applications

• Application: WB ; FACS ; ELISA

Handling

• Format: Liquid
• Unit size: 100 ug
• Shipping conditions: Dry ice

References

• Sandoval et al. 2006. Exp Cell Res. 312(13):2465-75. PMID: 16730350.