Cat. #151319
Anti-ASF1 [ASF 4A1/3]
Cat. #: 151319
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: Anti-Silencing Function 1 (ASF1)
Class: Monoclonal
Application: WB
Reactivity: Human ; Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-ASF1 [ASF 4A1/3]
- Research fields: Genetics
- Clone: ASF 4A1/3
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human ; Mouse
- Host: Mouse
- Application: WB
- Description: Anti-silencing function 1 (Asf1) belongs to the H3/H4 family of histone chaperones. Its function has not been fully elucidated but it is believed to be involved in ensuring a constant supply of histones at sites of nucleosome assembly. Asf1 interacts with the DNA damage checkpoint protein Rad53 and so may have a role in cell cycle regulation and DNA responses. ASF1 A and B (the two human subtypes) are the only known substrate for Tousled-like kinase (TLK). The Tousled kinases are themselves regulated by the checkpoint protein kinases involved in the DNA damage response.
- Immunogen: FLAG-ASF1A
- Isotype: IgG2a
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Anti-Silencing Function 1 (ASF1)
- Target background: Anti-silencing function 1 (Asf1) belongs to the H3/H4 family of histone chaperones. Its function has not been fully elucidated but it is believed to be involved in ensuring a constant supply of histones at sites of nucleosome assembly. Asf1 interacts with the DNA damage checkpoint protein Rad53 and so may have a role in cell cycle regulation and DNA responses. ASF1 A and B (the two human subtypes) are the only known substrate for Tousled-like kinase (TLK). The Tousled kinases are themselves regulated by the checkpoint protein kinases involved in the DNA damage response.
Applications
- Application: WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Sillj et al. 2001. Curr Biol. 11(13):1068-73. PMID: 11470414.
- Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases.
