Cell line FAQs Page

[…] lines Consider how much time, money and effort were spent in developing or obtaining your cell culture: Many widely used cell lines can be purchased for a few hundred dollars/pounds/euros per […]

Cell Culture Media FAQs Page

Cell culture media Plasmax Cell culture media Batch numbers and expiration dates are provided with cell culture products, including media, to ensure quality, stability and full traceability for records in […]

Organoid FAQs Page

[…] reduced (GFR) matrigel, adherence to proper passaging techniques and sourcing high quality organoid-specific media and cell culture reagents.     Patient-derived organoids can be established from both healthy and tumour tissue biopsies […]

PlasmaxTM: A physiologically relevant cell culture media Blog Post

PlasmaxTM is a physiologically relevant cell culture medium that closely resembles the metabolic and nutritional profile of human plasma. Unlike traditional media designed to supply excessive levels of a few nutrients, […]

Plasmid map for pcPPT-mPGK-attR-sLPmCherry-WPRE vector

pcPPT-mPGK-attR-sLPmCherry-WPRE vector Product

[…] cloned upstream of the mCherry gene, under the control of a mouse PGK promoter (sLPmCherry). Cell lines stably transfected with the plasmid express and release a cell-penetrating fluorescent protein, which […]

MB49 Cell Line in culture - sub confluence (A)

MB49 Cell Line Product

MB49 cell line used as an in vitro and in vivo model of bladder cancer, with enhanced metastatic potential for further migratory investigation.

Dr. Wytske M. van Weerden and CancerTools.org Blog Post

[…] interest, Dr. van Weerden and her team have generated a series of 38 prostate cancer cell lines which are now available through the CancerTools.org collection. Prostate cancer is the 2nd […]

PlasmaxTM vs DMEM the impact of physiologically relevant cell culture media Blog Post

Choosing an appropriate cell culture medium is a crucial step in in vitro cell biology research. With a wide variety of media currently available, finding the correct one for your cell type and particular experiment can be challenging. Sunada Khadka, a PhD Candidate at MD Anderson Cancer Center, studies cancer metabolism in glioma cells. Glioma is an intra-axial brain tumour which originates in the glial cells that surround and support neurons in the brains. During her latest research on anaplerosis in glioma cells, Sunada’s initial results obtained in vitro using traditional medium were not reproduced in her in vivo experiments. This led to additional time and resources being used to try and understand the discrepancy. Here, we explore Sunada’s latest research, and the role PlasmaxTM, a physiologically relevant media, played in resolving the discrepancy between her in vitro and in vivo experimental results. The researcher Sunada Khadka PhD candidate, MD Anderson Cancer Center Sunada’s research explored the possibility of synergistically killing tumour cells through the inhibition of glycolysis and glutaminolysis, two metabolic pathways that feed The Citric Acid (TCA) cycle. A novel enolase inhibitor, HEX, was used as a glycolysis inhibitor in this study. HEX was developed through the concept of collateral lethality wherein the passenger deletion of the glycolytic gene ENO1 within a subset of gliomas, selectively renders cancer cells sensitive to inhibition of the redundant isoform ENO2. HEX was tested in combination with CB-839. CB-839 is a glutaminase inhibitor which targets glutamine metabolism and is currently being investigated in randomised clinical trials against a range of malignancies. This made CB-839 of primary interest to extend the metabolism-targeted therapy. Initially, a pyruvate-free traditional media (DMEM) was used for the in vitro experiments which suggested a very strong effect of CB-839 on ENO1-deleted cancer cells. The combination of CB-839 and HEX provided a dramatic synergetic effect that seemed specific to ENO1-deleted cells. However, when it was attempted to recapitulate the in vitro results in vivo, within an intracranial tumour model, no effect with CB-839 alone and no additive effects with HEX could be seen. As CB-839 is known to be very poorly permeable across the brain, a subcutaneous in vivo tumour model was used, where Blood Brain Barrier penetration is not an issue. In this case some delay in tumour growth was observed after using CB-839 alone and when used in combination with HEX, but not to the extent seen in the in vitro research. Figure 1. ENO1-deleted glioma cells (D423) were implanted intracranially in immunocompromised nude mice and tumor growth was monitored weekly across different treatment groups by T2 MRI (indicated by dashed yellow outlines) 20-30 days after tumor implantation. Khadka et al. 2021. This inconsistency in data led to a return to in vitro experimental conditions and a closer examination of the cell culture media used. PlasmaxTM was selected as a cell culture media that better reflected the in vivo nutrient profile. PlasmaxTM is a ready-to-use, physiologically relevant cell culture medium, consisting of >80 components, of which >50 have been optimised to levels found within human plasma. By comparing the  in vitro results from PlasmaxTM to DMEM, it was observed that the toxicity of CB-839 in […]

Melan-a Cell Line Product

Melan-a cell line with use to investigate melanoma skin cancer.