Cat. #161935
SK-MES-1 Cell Line
Cat. #: 161935
Availability: 8-10 weeks
Tissue: Lung
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. 58.2.209. PMID: 833871.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-MES-1 Cell Line
- Cancer: Skin cancer
- Tissue: Lung
- Donor: This cell line was established in 1970 from a metastatic site (pleural effusion) in a 65-year-old Caucasian male with squamous cell carcinoma of the lung.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 1-2 PGM3, 1
- Description: SK-MES-1 is a human lung cancer cell line that displays epithelial morphology and grows as monolayers in tissue culture.
- Application: 3D cell culture, Cancer research
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood Type O; Rh+; HLA A3, Aw30, B7, B27
Applications
- Application: 3D cell culture, Cancer research
Handling
- Growth medium: 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium then supplemented with fetal bovine serum to a final concentration of 10%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: X,YCSF1PO: 12D13S317: 11D16S539: 13D5S818: 11D7S820: 8TH01: 6,9.3TPOX: 8vWA: 14D3S1358: 16D21S11: 29,30Penta_E: 5,11Penta_D: 12,13D8S1179: 13,14FGA: 20,24D19S433: 14D2S1338: 18,19D18S51: 17
References
- Blobel et al. 1984. Virchows Arch B Cell Path Incl Mol Pathol. 45(4):407-429. PMID: 6203212.
