MCF10A EditR (Constitutive Cas9 expression) cell line

cells that stably express Cas9 nuclease

Contributors

Inventor Institute
Corrado Santocanale National University of Ireland Galway
Cat. #: 161756
Research Fields: Cancer
Organism: Human
Tissue: Breast
Gender: Female
Growth properties: Adherent
Primary citation: Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864
Product description: MCF10AEditR cells that stably express Cas9 nuclease. The cell line is used to perform a CRISPR-Cas9 genome-wide screen to identify genes that, when lost, promote the proliferation of cells treated with sub-efficacious doses of a CDC7i
Gender: Female
CRISPR: Yes
Production details: MCF10AEditR cells that stably express Cas9 nuclease were generated by lentiviral transduction of the Edit-R Cas9 plasmid and blasticidin-mediated selection.
Parental cell line: MCF10A
Model description: CRISPR
disease: Fibrocystic disease
Products or characteristics of interest: CRISPR drug screening
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM supplemented with 5% Horse serum, 100 ng/ml cholera toxin, 10 ?g/ml insulin, 20 ng/ml epidermal growth factor (Peprotech), 500 ng/ml hydrocortisone, 50 U/ml penicillin and 50 ?g/ml Streptomycin
Temperature: 37° C
Atmosphere: 5% CO2
Biosafety level: 1
References:

Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864

Related Tools for Cell lines

Cat. # Tool Name
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151626 A375P beta6-puro Cell Line Key Info View Tool
151630 PDVC57B Cell Line Key Info View Tool
151631 C-Raf KI D486A Cell Line Key Info View Tool
151655 FLYA-13 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.