MCF10A EditR (Constitutive Cas9 expression) cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: MCF10A EditR (Constitutive Cas9 expression) cell line
• Research fields: Cancer
• Parental cell: MCF10A
• Organism: Human
• Gender: Female
• Tissue: Breast
• Disease: Fibrocystic disease
• Growth properties: Adherent
• Model description: CRISPR
• Crispr: Yes
• Products or characteristics of interest: CRISPR drug screening
• Description: MCF10AEditR cells that stably express Cas9 nuclease. The cell line is used to perform a CRISPR-Cas9 genome-wide screen to identify genes that, when lost, promote the proliferation of cells treated with sub-efficacious doses of a CDC7i
• Application: SDS-PAGE and western blotting
• Production details: MCF10AEditR cells that stably express Cas9 nuclease were generated by lentiviral transduction of the Edit-R Cas9 plasmid and blasticidin-mediated selection.
• Biosafety level: 1

Applications

• Application: SDS-PAGE and western blotting

Handling

• Volume: Frozen
• Growth medium: DMEM supplemented with 5% Horse serum, 100 ng/ml cholera toxin, 10 ?g/ml insulin, 20 ng/ml epidermal growth factor (Peprotech), 500 ng/ml hydrocortisone, 50 U/ml penicillin and 50 ?g/ml Streptomycin
• Temperature: 37° C
• Atmosphere: 5% CO2
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Cultured in antibiotics: Penicillin / streptomycin

References

• Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864