DLD-1/EGFP_LC3 – Mito DsRed

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: DLD-1/EGFP_LC3 – Mito DsRed
• Cancer: Digestive / Gastrointestinal cancer
• Cancers detailed: Colorectal adenocarcinoma
• Research fields: Cancer;Disease (not cancer);Metabolism
• Parental cell: DLD-1
• Organism: Human
• Gender: Male
• Tissue: Colon
• Disease: Cancer
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Transgenic
• Crispr: No
• Conditional: No
• Description: Colon cancer cell line DLD-1 stably expressing EGFP-LC3 and Mito DsRed for use as a mitophagy sensor.

Handling

• Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated), or RPMI 1640 with 2mM L-Glutamine and 10 Fetal Bovine serum.
• Temperature: 37° C
• Atmosphere: 5% CO2
• Shipping conditions: Dry Ice
• Storage medium: Growth medium + 10% DMSO
• Storage conditions: Liquid Nitrogen
• Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?‚°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
• Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm?‹Â› .
• Cultured in antibiotics: No

References

• Lupitha SS, et.al. PMID: 32109534.