Cat. #157680
Ln12 TCR Tg mice
Cat. #: 157680
Sub-type: Mouse
Availability: 6-8 weeks
Model: Transgenic
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Contributor
Inventor: Thorbald Van Hall
Institute: Leiden University and Leiden University Medical Center ; Stichting Oncode Institute
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: Ln12 TCR Tg mice
- Tool sub type: Mouse
- Model: Transgenic
- Conditional: No
- Conditional description: The expression of the TCR is driven by the CD2 promoter and not conditional
- Description: Tumor cells frequently escape from CD8+ T cell recognition by abrogating MHC-I antigen presentation. Deficiency in processing components, like the transporter associated with antigen processing (TAP), results in strongly decreased surface display of peptide/MHC-I complexes. A class of hidden self-antigens known as T cell epitopes associated with impaired peptide processing (TEIPP), which emerge on tumor cells with such processing defects can be investigated with this model.
Using this mouse model, it is possible to investigate the generation of the TEIPP T cell repertoire specifically. These animals harbour rearranged receptors recognizing the Ln12 complex, recognizing a peptide in the context of the non-classical MHC class I molecule Qa-1b (encoded by the H2-T23 gene). The mouse model enables thymus selection and the study of emerging na
ve CD8+ T cells. Author publication: PMID: 29422902 - Genetic background: The Ln12 TCR-transgenic mouse strain was generated by transgenesis of the TCRa and TCR
genes of the Ln12 T cell clone. The TCRa and TCR chains were separately cloned into pCRII-TOPO plasmid vectors using RT-PCR and sequenced. Expression of TCRs was performed by retroviral transduction of the TCR genes in C57BL/6 splenocytes using pMX vector. For transgenesis, the two chains were separately cloned into VA-hCD2 vectors and inserts were subsequently injected in C57BL/6 oocytes. (PMID: 29422902) - Zygosity: Unknown
- Strain: C57BL/6
- Production details: The Ln12 TCR-transgenic mouse strain was generated by transgenesis of the TCRι and TCRβ genes of the Ln12 T cell clone. The TCRι and TCRβ chains were separately cloned into pCRII-TOPO plasmid vectors using RT-PCR and sequenced. Expression of TCRs was performed by retroviral transduction of the TCR genes in C57BL/6 splenocytes using pMX vector. For transgenesis, the two chains were separately cloned into VA-hCD2 vectors and inserts were subsequently injected in C57BL/6 oocytes. (PMID: 29422902)
- Breeding information: These mice breed normally in heterozygous or homozygous background
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Ln12 TCR
References
- Doorduijn et al. 2018. Front Immunol. 9:60. PMID: 29422902.
