Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6]

Exclusive Tool

Contributors

Inventor Institute
Jonathon Pines University of Cambridge
SKU: 153463
Product description: Somatic knock-in reporter cell line with endogenous expression of Cyclin A2 fused to the yellow fluorescent protein Venus (Cyclin A2-Venus fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin A binds and activates CDC2 or CDK2 kinases, and thus this cell line functions as a reporter for both cell cycle G1/S and G2/M transitions. See also the Mad2 and Cyclin B1 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for Cyclin A2 levels and activity.
Alternate name: CCNA2
Conditional: No
Production details: To generate the cell line recombinant adenovirus-associated virus (rAAV)-mediated gene targeting was used to introduce the open reading frame (ORF) of yellow fluorescent protein (Venus) into the last exon of one allele of the CCNA2 (Cyclin A2) gene in hTert-RPE1 cells (RPE1; retinal pigment epithelial). RPE1 cells were chosen because they have a normal diploid karyotype, are not transformed and exhibit little cell death when arrested in mitosis; tagging the endogenous Cyclin A2 protein in RP…
Parental cell line: RPE1
Cat. #: 153463
Provenance and Ownership: The Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6] is owned by Cancer Research UK. CancerTools supplies the Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology
Organism: Human
Tissue: Eye
Model: Reporter
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: F12/DMEM (Sigma: D6241) + 2mM GlutaMAX?„?‚ƒ?‚¤(Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37oC; 5% CO2
Mycoplasma free: Yes
Biosafety level: 1
References:

Collin et al., 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242

Images

View Gallery
Cyclin A2-Venus Reporter Cell line [RPE1 cycA2-venus/+ KI clone D6]

Related Tools for Cell lines

Cat. # Tool Name
153465 Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1] Key Info View Tool
153464 Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] Key Info View Tool
153466 Ruby-Mad2 Reporter Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.