Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11]
Contributors
Inventor
Institute
Jonathon Pines
University of Cambridge
SKU:
153464
Product description:
Somatic knock-in reporter cell with endogenous expression of cyclin B1 fused to the yellow fluorescent protein Venus (cyclin B1-Venus fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This Cyclin B1 cell line functions as a cell cycle reporter across the G2/mitotic-specific phase of the cell cycle. See also the Mad2 and Cyclin A2 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for cyclin B1 levels and activity.
Alternate name:
CCNB1
Conditional:
Yes
Production details:
To generate the cell line recombinant adenovirus-associated virus (rAAV)-mediated gene targeting was used to fuse the open reading frame (ORF) of yellow fluorescent protein (Venus) with exon 9 of the CCNB1 (Cyclin B1) gene in hTert-RPE1 cells (RPE1; retinal pigment epithelial). RPE1 cells were chosen because they have a normal diploid karyotype, are not transformed and exhibit little cell death when arrested in mitosis; tagging the endogenous Cyclin B1 protein in RPE1 cells avoided the compl…
Parental cell line:
RPE1
Cat. #:
153464
Provenance and Ownership:
The Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] is owned by Cancer Research UK. CancerTools supplies the Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
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![Cyclin B1-Venus Reporter Cell line [RPE1 cycB1-venus/+ KI clone 20G11] Metaphase; example 1](https://cancertools.org/wp-content/uploads/7acd79a2-1091-4168-8dcf-1f83ae2cb8f2-1.png)
