Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1]

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Contributors

Inventor Institute
Jonathon Pines University of Cambridge
SKU: 153465
Product description: Somatic knock-in reporter cell line with endogenous expression of Mad2 fused to the yellow fluorescent protein Venus (Venus-Mad2 fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cell line functions as a cell cycle reporter across the metaphase-to-anaphase transition of the cell cycle. See also the Cyclin A2 and Cyclin B1 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for Mad2 levels and activity.
Alternate name: MAD2L1
Conditional: No
Production details: To generate the cell line recombinant adenovirus-associated virus (rAVV)-mediated gene targeting was used to fuse the yellow fluorescent protein (Venus) ORF to the 5′ of exon 1 of one allele of the MAD2L1 gene. The Mad2 was tagged at its N-terminus because a GFP-Mad2 fusion protein had previously been demonstrated to be functional in Drosophila.
Parental cell line: RPE1
Related tools: Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] ; Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6] ; Ruby-Mad2 Reporter Cell Line
Cat. #: 153465
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology
Organism: Human
Tissue: Eye
Model: Reporter
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: F12/DMEM (Sigma: D6241) + 2mM GlutaMAX?(Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37?‚°C; 5% CO2.
Mycoplasma free: Yes
Biosafety level: 1
References:

Collin et al., 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242

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Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1] Metaphase

Related Tools for Cell lines

Cat. # Tool Name
153463 Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6] Key Info View Tool
153464 Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11] Key Info View Tool
153466 Ruby-Mad2 Reporter Cell Line Key Info View Tool

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