Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1]
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Contributors
Inventor
Institute
Jonathon Pines
University of Cambridge
SKU:
153465
Product description:
Somatic knock-in reporter cell line with endogenous expression of Mad2 fused to the yellow fluorescent protein Venus (Venus-Mad2 fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cell line functions as a cell cycle reporter across the metaphase-to-anaphase transition of the cell cycle. See also the Cyclin A2 and Cyclin B1 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for Mad2 levels and activity.
Alternate name:
MAD2L1
Conditional:
No
Production details:
To generate the cell line recombinant adenovirus-associated virus (rAVV)-mediated gene targeting was used to fuse the yellow fluorescent protein (Venus) ORF to the 5′ of exon 1 of one allele of the MAD2L1 gene. The Mad2 was tagged at its N-terminus because a GFP-Mad2 fusion protein had previously been demonstrated to be functional in Drosophila.
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![Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1] Metaphase](https://cancertools.org/wp-content/uploads/d403407e-097f-4c1e-9795-64bd69ec8ac3-1.png)
