H413 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: H413 Cell Line
• Research fields: Cancer
• Tissue: Buccal mucosa
• Disease: Cancer
• Growth properties: Adherent
• Model: Tumour line
• Description: Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a 53 year-old female patient. STNMP stage II, moderately differentiated, node negative tumour. Mutant p53, codon 68 exon 4, G to T; wild type K-, N- and Ha-ras. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth
• Cellosaurus id: CVCL_2465

Target Details

• Target: Human oral squamous cell carcinoma, buccal mucosa

Handling

• Format: Frozen
• Growth medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Subculture routine: Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density of 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability.

References

• Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
• Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
• Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
• TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
• Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
• Epidermal growth f...