HCT 116 ADAM17 Cell Line

HCT 116 ADAM17 Cell Line showed that overexpression of ADAM-17 significantly decreases the effect of chemotherapy on tumor growth and apoptosis.

Contributors

Inventor Institute
Sandra Van Schaeybroeck Queen's University Belfast
Cat. #: 153209
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Colon
Model: Knock-In
Growth properties: Invasion, migration
Cancer Types In Detail: Colorectal
Product description: Chemotherapy (5-fluorouracil) treatment has been shown to result in acute increases in transforming growth factor-a, amphiregulin, and heregulin ligand shedding in vitro and in vivo correlating with significantly increased ADAM-17 activity. Small interfering RNAmediated silencing and pharmacologic inhibition confirmed that ADAM-17 was the principal ADAM involved in this prosurvival response. HCT 116 ADAM17 Cell Line showed that overexpression of ADAM-17 significantly decreases the effect of chemotherapy on tumor growth and apoptosis.
Conditional: No
Production details: HCT116 cells were cotransfected with 10 ÎĚ?Ÿg of a plasmid containing hemagglutinin (HA)-tagged full-length mouse ADAM-17 (HA-ADAM-17) and a construct expressing a puromycin resistance gene. Stably transfected cells were selected and maintained in medium supplemented with 1 ÎĚ?Ÿg/mL puromycin.
Additional notes: Offered under licence from the Spanish National Research Council (CSIC)
Cellosaurus ID: CVCL_HG03
Parental cell line: HCT 116
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
Recommended controls: HCT 116 parental line
References:

Kyula et al. 2010. Clin Cancer Res. 16(13):3378-89. PMID: 20570921.

Chemotherapy-induced activation of ADAM-17: a novel mechanism of drug resistance in colorectal cancer.

Related Tools for Cell lines

Cat. # Tool Name
151014 MCF7 AREc32 Cell Line Key Info View Tool
151446 FLYRD18 Cell Line Key Info View Tool
151450 JIM3 Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool
151626 A375P beta6-puro Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.