BICR 18 Cell Line

Adherent keratinocyte cell line derived from a lymph node metastasis squamous cell carcinoma (SSC) of the larynx. Keratin and involucrin markers present.

Contributors

Inventor Institute
Eric Kenneth Parkinson Cancer Research UK, Glasgow: The Beatson Institute
Cat. #: 152843
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Cancer types: Head and neck cancer;Head and neck cancer
Organism: Human
Tissue: Larynx
Gender: Male
Model: Tumourigenic cell line
Growth properties: Adherent
Cancer Types In Detail: Larynx squamous cell carcinoma (lymph node metastasis);Larynx squamous cell carcinoma (lymph node metastasis)
Alternate name: bicr18; BICR-18; BICR18; Beatson Institute for Cancer Research 18; HNSCC; SCC-HN
Product description: Adherent keratinocyte cell line derived from a lymph node metastasis squamous cell carcinoma (SSC) of the larynx. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
Gender: Male
CRISPR: Yes
Conditional: Yes
Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 8,13 D16S539: 12,14 D5S818: 12 D7S820: 9 THO1: 7 TPOX: 9,12 vWA: 17,20
Cellosaurus ID: CVCL_2309
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 µg/ml Hydrocortisone.
Subculture routine: Split sub-confluent cultures (70-80%) 1:2 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density of 4 x 10,000 cells/cm². Use of conditioned medium at a ratio of 1:1 with fresh medium can improve cell condition. Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells. Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37°C water bath and the contents quickly transferred to a 15ml centrifuge tube. DMEM medium is added drop wise to 5ml. Cells are centrifuged at 150 x g for 5 minutes at Room Temperature. Cells are resuspended in 5ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 10^4 cells/cm².
Mycoplasma free: Yes
References:

Loughran et al. 1997. Oncogene. 14(16):1955-1964. PMID: 9150362.

Edington et al. 1995. Mol Carcinog. 13(4):254-265. PMID: 7646764.

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