MCF7/TAMR-8 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: MCF7/TAMR-8 Cell Line
• Alternate name: MCF-7/TAMR-8; TAMR-8
• Cancer: Breast cancer
• Cancers detailed: Breast cancer;Tamoxifen resistant
• Research fields: Cancer;Drug development
• Tool sub type: Continuous
• Parental cell: MCF7 S0.5
• Organism: Human
• Gender: Female
• Tissue: Breast
• Disease: Cancer
• Model: Tumour line
• Conditional: Yes
• Description: MCF7/TAMR-8 cell line is a stable tamoxifen-resistant subline. This cell line has been established in tissue culture after long term treatment with 1 uM tamoxifen. Tamoxifen (Nolvadex) is a widely used drug for hormone-dependent cancer. Tamoxifen resistance (either primary or acquired) makes oestrogen receptor-positive breast cancer much more difficult to treat. This cell line was produced from the parental cell line MCF7/S0.5, as a model cell system to study the effects of tamoxifen resistant cancer growth. Tamoxifen-resistant cells are passaged continuously in the presence of 1 uM tamoxifen. This concentration is lethal for the parental cell line. MCF7/TAMR-8 cells are oestrogen receptor positive and progesterone receptor negative. Previous applications of this cell line include treatment with steroidal antioestrogens. MCF7/TAMR-8 enables identification of new hormone therapies and greater understanding of the signalling pathways/methods behind tamoxifen resistance. Additionally MCF/TAMR-8 can aid in identifying new predictive markers for response to hormonal therapy.
• Application: Investigate signalling pathways involved in tamoxifen
• Production details: The parental cell line for the MCF7/TAMR-8 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-8 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line.
• Biosafety level: 1
• Additional notes: MCF7/TAMR-8 cells are oestrogen receptor positive and progesterone receptor negative. MCF7/TAMR-8 cells are growth inhibited by the pure antioestrogen fulvestrant. The oestrogen receptor is a major driver of growth of MCF7/TAMR-8 cell.
• Recommended controls: MCF7-S0.5 parental line
• Cellosaurus id: CVCL_1D44

Target Details

• Target: Oestrogen receptor

Applications

• Application: Investigate signalling pathways involved in tamoxifen
• Application notes: MCF7/TAMR-8 cells are oestrogen receptor positive and progesterone receptor negative. MCF7/TAMR-8 cells are growth inhibited by the pure antioestrogen fulvestrant. The oestrogen receptor is a major driver of growth of MCF7/TAMR-8 cell. Passage 398 (AL2687, AL2688)

Handling

• Format: Frozen
• Passage number: Passage 398 (AL2687, AL2688)
• Growth medium: Phenol red-free DMEM:Ham's F-12 containing 1% fetal bovine serum, 2 mM Glutamax and 6 ng/ml insulin. To maintain high-level resistance, medium was supplemented with Tamoxifen (1 uM).
• Temperature: 37° C
• Atmosphere: 5% CO2
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

Related Tools

• Related tools: MCF7/S0.5 Cell Line

References

• Thrane et al. 2015. Oncogene. 34(32):4199-210. PMID: 25362855.
• Elias et al. 2015. Oncogene. 34(15):1919-1927. PMID: 24882577.
• Pedersen et al. 2014. Int J Oncol. 45(5):2167-2175. PMID: 25175082.
• Lundqvist et al. 2014. Steroids. 85:30-35. PMID: 24747771.
• Thrane et al. 2013. Breast Cancer Res Treat. 139(1):71-80. PMID: 23609470.
• Larsen et al. 2012. Int J Oncol. 41(5):1863-1870. PMID: 22961366.
• Cutrupi et al. 2012. Oncogene. 31(40):4353-4361. PMID: 22249258.
• Frankel et al. 2007. Bre...