Cat. #151646
ATF2 Deleter Mouse
Cat. #: 151646
Sub-type: Mouse
Availability: 8-10 weeks
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Nic Jones
Institute: Cancer Research UK, Manchester Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: ATF2 Deleter Mouse
- Research fields: Cell biology;Cell signaling and signal transduction;Developmental biology;Genetics;Immunology
- Tool sub type: Mouse
- Conditional: No
- Description: Deletion of ATF2 DNA binding domain. Transcription factor (BZIP-type, AP1-family).
- Genetic background: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library (RZPD), cloned into pPGKneobpAlox2PGKD, transfected into 129/ola embryonic stem cells. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.
- Phenotype: Homozygous postnatal lethality, heterozygous normal phenotype
- Zygosity: Heterozygous
- Production details: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library (RZPD), cloned into pPGKneobpAlox2PGKD, transfected into 129/ola embryonic stem cells. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: ATF2
References
- Breitwieser et al. 2007. Genes Dev. 21(16):2069-82. PMID: 17699753.
- Feedback regulation of p38 activity via ATF2 is essential for survival of embryonic liver cells.
