Raf-1FF/FF knockin mouse

A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling;

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
SKU: 151477
Product description: A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling;
Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
Additional notes: Genetic Background / Cross History : C57BL/6
Cat. #: 151477
Research Fields: Cancer; Cell signaling and signal transduction; Genetics; Neurobiology
Target: Raf1 Y340F Y341F (inactive mutant)
Disease: Cancer
Model: Knock-In
Strain: C57BL/6
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.

MEK kinase activity is not necessary for Raf-1 function.

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