Cat. #151559
MEF UNG KO Cell Line
Cat. #: 151559
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Mouse
Tissue: Embryo
Disease: Cancer
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Thomas Lindahl
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MEF UNG KO Cell Line
- Cancer: Blood cancer
- Cancers detailed: B cell lymphoma
- Research fields: Genetics
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer
- Model: Knock-Out
- Description: The MEF UNG KO Cell Line is a tool for in vivo study of the function of UNG and the effects of DNA mismatch during DNA synthesis. UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway.
- Production details: Primary MEFs were prepared from embryos, and permanent cell lines established from transformed clones arising spontaneously after repeated passage in culture, by standard procedures
Target Details
- Target: Uracil-DNA Glycosylase (UNG)
Handling
- Format: Frozen
- Growth medium: DMEM:F12 (1:1) + 10% FCS.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
References
- Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.
- Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.
