Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1]

This antibody is a useful marker of apicobasal polarity in the epithelium.

Contributors

Inventor Institute
Cecile Wright-Crosnier ; Julian Lewis ; Linda Ariza-McNaughton ; Neil Vargesson Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 151515
Product description: This antibody is a useful marker of apicobasal polarity in the epithelium.
Conjugation: Unconjugated
Isotype: IgG1
Molecular weight: 170 kDa
Immunogen: Lysate of zebrafish intestine
Myeloma used: Sp2/0-Ag14
Cat. #: 151515
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cell biology; Developmental biology; Tags and cell markers
Application: IHC ; IHC ; IF ; WB
Target: Zebrafish Basolateral Pole of Cells
Reactivity: Zebrafish
Clone: FIS 2H9/1
Host: Mouse
Class: Monoclonal
Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. Antibody 2H9 recognises a membrane-associated epitope on the gut epithelial cells, concentrated basolaterally. In one month old zebrafish it appears especially prominent in the basal parts of the elongated cells of the villi.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

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Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1]

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