Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2]

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Monoclonal antibody which labels all secretory cells within the zebrafish intestinal epithelium.

Contributors

Inventor Institute
Cecile Wright-Crosnier ; Julian Lewis ; Linda Ariza-McNaughton ; Neil Vargesson Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 151483
Product description: Monoclonal antibody which labels all secretory cells within the zebrafish intestinal epithelium. Subsequent studies have reported that the 2F11 monoclonal antibody recognises Annexin A4 (Anxa4) in zebrafish.
Conjugation: Unconjugated
Isotype: IgG1
Molecular weight: 170 kDa, 120 kDa, 90 kDa and 35 kDa
Immunogen: Lysate of zebrafish intestine
Myeloma used: Sp2/0-Ag14
Cat. #: 151483
Provenance and Ownership: The Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2] is owned by Cancer Research UK. CancerTools supplies the Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cell biology; Cell signaling and signal transduction; Developmental biology; Genetics; Stem cell biology; Tags and cell markers
Application: IHC ; ELISA ; IHC ; IF ; IP ; WB
Target: Zebrafish gut secretory cell epitopes
Reactivity: Zebrafish
Clone: FIS 2F11/2
Host: Mouse
Class: Monoclonal
Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels all the gut secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants. The antibody also labels hepatopancreatic ducts, intrahepatic bile ducts and enteroendocrine. Anti-2F11 provides an early marker to distinguish cell types and cell polarity in the zebrafish gut.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Nissim et al. 2019. Nat Genet. 51(9):1308-1314. PMID: 31406347.

Gao et al. 2019. J Mol Cell Biol. 11(6):448-462. PMID: 30428031.

Murdoch et al. 2019. PLoS Pathog. 15(3):e1007381. PMID: 30845179.

Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268.

Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268.

Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244.

Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244.

Tom et al. 2015. Sci Rep. 5:14384. PMID: 26399509.

Kimmel et al. 2015. Sci Rep. 5:14241. PMID: 26384018.

Wang et al. 2015. Dev Dyn. 244(6):724-35. PMID: 25773748.

Ningappa et al. 2015. PLoS One. 10(9):e0138381. PMID: 26379158.

Flasse et al. 2013. Dev Biol. 376(2):187-97. PMID: 23352790.

Palha et al. 2013. PLoS Pathog. 9(9):e1003619. PMID: 24039582.

Garnaas et al. 2012. Dev Biol. 372(2):178-89. PMID: 22982668.

Manfroid et al. 2012. Dev Biol. 366(2):268-78. PMID: 22537488.

Ninov et al. 2012. Development. 139(9):1557-67. PMID: 22492351.

Poulain et al. 2011. Development. 138(16):3557-68. PMID: 21771809.

Shin et al. 2011. Development. 138(7):1339-48. PMID: 21385764.

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.

Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.

Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

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Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2]

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