Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]

This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.

Contributors

Inventor Institute
Cecile Wright-Crosnier ; Julian Lewis ; Linda Ariza-McNaughton ; Neil Vargesson Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151512
Tool sub type: Primary antibody
Unit size: 100 ug
Cancer types: Colorectal cancer
Research Fields: Cell biology;Cell signaling and signal transduction;Developmental biology;Genetics;Stem cell biology;Tags and cell markers
Application: IHC ; IP ; WB
Target: Zebrafish gut absorptive cell epitopes
Reactivity: Zebrafish
Clone: FIS 4E8/1; 2F11
Host: Mouse
Class: Monoclonal
Product description: This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
Conjugation: Unconjugated
Isotype: IgG1
Molecular weight: 200 kDa
Immunogen: Lysate of zebrafish intestines
Myeloma used: Sp2/0-Ag14
Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyxof intestinal absorptive cells (apical ends of microvilli).
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.

Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.

Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

Images

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Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]
Adapted from Matthews et al. 2008. Dev Dyn. 237(1)

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