Anti-CD79b [B29/123] mAb

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD79b [B29/123] mAb
• Alternate name: CD79b Molecule; CD79B Antigen (Immunoglobulin-Associated Beta); Immunoglobulin-Associated B29 Protein; B-Cell-Specific Glycoprotein B29; Ig-Beta
• Research fields: Cell signaling and signal transduction;Immunology
• Clone: B29/123
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: IHC ; WB
• Description: The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling.
• Immunogen: Synthetic peptide representing the c-terminus from residue 215 of the murine B29 polypeptide.
• Isotype: IgG2b
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: CD79b
• Target background: The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling.

Applications

• Application: IHC ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Hauser et al. 2013. Mol Immunol. 54(3-4):247-53. PMID: 23318223.
• Broad feedback inhibition of pre-B-cell receptor signaling components.
• Ouchida et al. 2010. J Immunol. 185(1):294-301. PMID: 20519653.
• A role for lysosomal-associated protein transmembrane 5 in the negative regulation of surface B cell receptor levels and B cell activation.
• Lankester et al. 1994. J Immunol. 152(5):2157-62. PMID: 8133032.
• Evidence for a direct physical interaction of membrane IgM, IgD, and IgG with the B29 gene product.
• Jones et al. 1993. J Immunol. 150(12):5429-35. PMID: 8515069.
• Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
• Brouns et al. 1993. Eur J Immunol. 23(5):1088-97. PMID: 8477803.
• The structure of the mu/pseudo light chain complex on human pre-B cells is consistent with a function in signal transduction.
• Mason et al. 1992. Eur J Immunol. 22(10):2753-6. PMID: 1396979.
• The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation.