Anti-Integrin alpha V [P2W7] mAb

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Integrin alpha V [P2W7] mAb
• Alternate name: Anti-Integrin antibody; P2W7; anti-alphav; anti-av
• Research fields: Stem cell biology
• Clone: P2W7
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 150-160 kDa
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF ; IP ; WB
• Description: Anti-Integrin alpha V [P2W7] is a monoclonal antibody which detects the ectodomain of integrin alpha-V(aV). Cell-matrix interactions are mediated primarily by integrins. Integrins have been implicated in cancer progression and metastasis. These integrins are composed of alpha and beta subunits to form a complete signaling molecule. Integrin aV is found in osteoclasts, placenta, melanoma cell lines and some epithelial cell lines. Expression of integrin aV increases with melanoma progression. Studies have shown P2W7 antibody-induced changes in cell morphology (ZR75-1 cells). The morphological changes noted were transient island formation.
• Immunogen: Ocular melanoma cell line (V+B2) a clone expressing high levels of alpha-v, beta-1
• Immunogen uniprot id: P06756
• Isotype: IgG1
• Recommended controls: A375M melanoma

Target Details

• Target: Integrin alpha V (CD51)
• Molecular weight: 150-160 kDa
• Tissue cell line specificity: A375M melanoma
• Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin aV associates with integrin b3 to form the osteoclast Fn antigen (vitronectin receptor). The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF Integrin aV is found in osteoclasts, placenta, melanoma cell lines and some epithelial cell lines. Expression of integrin aV increases with melanoma progression. Anti-integrin aV detects the ectodomain of human integrin alpha V/CD51.

Applications

• Application: FACS ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

• D'Agostino et al. 2020. Molecules. 25(3):. PMID: 31972968.
• Stantchev et al. 2019. Viruses. 11(10):. PMID: 31547585.
• Murase et al. 2017. J Neurosci Res. 95(12):2500-2510. PMID: 28509387.
• Richards et al. 2011. J Virol. 85(17):8766-8776. PMID: 21715474.
• Misselwitz et al. 2011. Mol Syst Biol. 7(1):474. PMID: 21407211.
• Schlippe et al. 2000. J Cell Sci. 113:425-437. PMID: 10639330.