MCF7 AREc32 Cell Line

AREc32: A stable human mammary MCF7-derived reporter cell line containing copies of the rat GST ARE linked to a luciferase gene.

Contributors

Inventor Institute
Roland Wolf University of Dundee
SKU: 151014
Product description: AREc32: A stable human mammary MCF7-derived reporter cell line containing copies of the rat GST ARE linked to a luciferase gene.
Conditional: Yes
Production details: The ARE-luciferase reporter plasmid was generated using the pGL3-promoter vector containing an SV40 promoter upstream of the firefly luciferase gene. They differ in the number of copies of ARE sequences that have been inserted, in head-to-tail orientation, through Nhe I & Xho I restrictionsites upstream of the promoter-luc+ transcriptional unit. A plasmid was made containing eight copies of the ARE (5'-GTGACAAAGCA-3', with the minimal functional sequence underlined) present in both rat GSTA2 and mouse gsta1; called pGL-8xARE. A linker with the sequence of 5'-CCC-3' and 5'-GGG-3' on the opposite strand was placed between individual cis-elements.pGL-8xARE, was stably transfected into MCF7 cells using the calcium phosphate method. Transfected cells were selected using 0.8 mg/mL G418 in the media for 3 to 4 weeks. The G418-resistant clones were isolated and screened by measuring their basal and inducible (obtained by treatment with 50 Amol/L t-BHQ) luciferase activities as described above. Positive clones, which showed low background and high inducible luciferase activity, were passaged and maintained in growth medium containing 0.8 mg/mL G418.
Cellosaurus ID: CVCL_1D32
Parental cell line: MCF7
Disease: Cancer
Cat. #: 151014
Provenance and Ownership: The MCF7 AREc32 Cell Line is owned by Cancer Research UK. CancerTools supplies the MCF7 AREc32 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing MCF7 AREc32 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Model: Reporter
Primary citation: Wang et al. 2006. Cancer Res. 66(22):10983-94. PMID: 17108137
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM with 2.5 mM Glutamax supplemented with 10% fetal bovine serum and antibiotics. Do not culture beyond 15 passages after revival.
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10^4 cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 days.
Recommended controls: MCF7 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Gameiro et al. 2017. Sci Rep. 7:45701. PMID: 28361919.

Discovery of the first dual GSK3? inhibitor/Nrf2 inducer. A new multitarget therapeutic strategy for Alzheimer's disease.

MacLeod et al. 2016. Br J Cancer. :. PMID: 27824809.

Aldo-keto reductases are biomarkers of NRF2 activity and are co-ordinately overexpressed in non-small cell lung cancer.

Basar et al. 2016. Phytochem Anal. 27(5):233-8. PMID: 27527356.

Utilization of the Ability to Induce Activation of the Nuclear Factor (Er…

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